ANTAGONISTS OF BOMBESIN/GASTRIN-RELEASING PEPTIDE INHIBIT GROWTH OF JAR HUMAN CHORIOCARCINOMA CELLS AND PRODUCTION OF CYCLIC-AMP IN-VITRO

  • Authors:
    • T ERTL
    • YF QIN
    • K GROOT
    • JE HORVATH
    • RZ CAI
    • AV SCHALLY
  • View Affiliations

  • Published online on: March 1, 1995     https://doi.org/10.3892/ijo.6.3.547
  • Pages: 547-553
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Abstract

The effects of bombesin/GRP antagonists RC-3095 and RC-3940-II on the in vitro proliferation of JAR human choriocarcinoma cells were evaluated. Antagonists RC-3095 and RC-3940-II effectively inhibited growth of cultured JAR cells, inducing a dose- and time-dependent decrease in the number of treated cells. RC-3940-II was more potent than RC-3095 in inhibiting the growth of JAR cells. Addition of RC-3940-II to JAR cell cultures significantly inhibited the cell proliferation at concentrations as low as 1 nM, while 10 nM RC-3095 was required for a similar effect. Receptor binding studies demonstrated the presence of a single class of binding sites for bombesin on JAR cells. RC-3940-II displaced [I-125]Tyr(4)-bombesin bound to the receptors. When JAR cells were cultured in the presence of 10 nM RC-3095 or RC-3940-II for 72 h, cAMP levels in the incubation medium were decreased by 70-80%, compared to the controls. These results suggest that bombesin/GRP antagonists RC-3095 and RC-3940-II inhibit the proliferation of JAR human chorionic adenocarcinoma cells in vitro and that these effects may involve intracellular cAMP pathway.

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March 1995
Volume 6 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
ERTL T, QIN Y, GROOT K, HORVATH J, CAI R and SCHALLY A: ANTAGONISTS OF BOMBESIN/GASTRIN-RELEASING PEPTIDE INHIBIT GROWTH OF JAR HUMAN CHORIOCARCINOMA CELLS AND PRODUCTION OF CYCLIC-AMP IN-VITRO. Int J Oncol 6: 547-553, 1995
APA
ERTL, T., QIN, Y., GROOT, K., HORVATH, J., CAI, R., & SCHALLY, A. (1995). ANTAGONISTS OF BOMBESIN/GASTRIN-RELEASING PEPTIDE INHIBIT GROWTH OF JAR HUMAN CHORIOCARCINOMA CELLS AND PRODUCTION OF CYCLIC-AMP IN-VITRO. International Journal of Oncology, 6, 547-553. https://doi.org/10.3892/ijo.6.3.547
MLA
ERTL, T., QIN, Y., GROOT, K., HORVATH, J., CAI, R., SCHALLY, A."ANTAGONISTS OF BOMBESIN/GASTRIN-RELEASING PEPTIDE INHIBIT GROWTH OF JAR HUMAN CHORIOCARCINOMA CELLS AND PRODUCTION OF CYCLIC-AMP IN-VITRO". International Journal of Oncology 6.3 (1995): 547-553.
Chicago
ERTL, T., QIN, Y., GROOT, K., HORVATH, J., CAI, R., SCHALLY, A."ANTAGONISTS OF BOMBESIN/GASTRIN-RELEASING PEPTIDE INHIBIT GROWTH OF JAR HUMAN CHORIOCARCINOMA CELLS AND PRODUCTION OF CYCLIC-AMP IN-VITRO". International Journal of Oncology 6, no. 3 (1995): 547-553. https://doi.org/10.3892/ijo.6.3.547