TRANSFORMATION BY V-ERB-B - INDUCTION OF APOPTOSIS IS ABROGATED IN A MYELOID CELL-LINE

  • Authors:
    • A DOLNIKOV
    • Y SHOUNAN
    • NJ HAWKINS
    • RL WARD
    • G SYMONDS
  • View Affiliations

  • Published online on: March 1, 1995     https://doi.org/10.3892/ijo.6.3.633
  • Pages: 633-638
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Abstract

Oncogene transformation represents a means to analyse events involved in myeloproliferation and leukemogenesis. We have previously shown that v-erb-B transforms a myeloid cell line (FDCP-1) to growth factor independence and acts to modulate the lineage of these cells allowing induction of erythroid differentiation. In this report we have compared apoptosis in parental and v-erb-B transformed FDCP-1 cells (FI v-erb-B). v-erb-B was found to protect these cells from apoptosis induced by IL-3 withdrawal, dexamethasone addition or serum withdrawal. In the case of IL-3 withdrawal, the suppression of apoptosis was reversed by herbimycin A, indicating that the effect was mediated through the tyrosine-kinase activity of the v-erb-B protein. The apoptotic protective effect exerted by v-erb-B for dexamethasone or serum withdrawal was seen despite growth suppression by these conditions. Taken together, these results indicate that transformation by this oncogene, in addition to its proliferation-enhancing properties, is due to its ability to inhibit apoptosis induced by growth factor withdrawal or growth suppression. The suppression of apoptosis induced by v-erb-B in hematopoietic cells appears to be important to the tumorigenic potential of this oncogene and may act as a means of in vivo tumor progression.

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March 1995
Volume 6 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
DOLNIKOV A, SHOUNAN Y, HAWKINS N, WARD R and SYMONDS G: TRANSFORMATION BY V-ERB-B - INDUCTION OF APOPTOSIS IS ABROGATED IN A MYELOID CELL-LINE. Int J Oncol 6: 633-638, 1995
APA
DOLNIKOV, A., SHOUNAN, Y., HAWKINS, N., WARD, R., & SYMONDS, G. (1995). TRANSFORMATION BY V-ERB-B - INDUCTION OF APOPTOSIS IS ABROGATED IN A MYELOID CELL-LINE. International Journal of Oncology, 6, 633-638. https://doi.org/10.3892/ijo.6.3.633
MLA
DOLNIKOV, A., SHOUNAN, Y., HAWKINS, N., WARD, R., SYMONDS, G."TRANSFORMATION BY V-ERB-B - INDUCTION OF APOPTOSIS IS ABROGATED IN A MYELOID CELL-LINE". International Journal of Oncology 6.3 (1995): 633-638.
Chicago
DOLNIKOV, A., SHOUNAN, Y., HAWKINS, N., WARD, R., SYMONDS, G."TRANSFORMATION BY V-ERB-B - INDUCTION OF APOPTOSIS IS ABROGATED IN A MYELOID CELL-LINE". International Journal of Oncology 6, no. 3 (1995): 633-638. https://doi.org/10.3892/ijo.6.3.633