THE FIRST INTRON OF HUMAN H-RAS IS REGULATED BY P53 - MEDIATION OF SPECIFIC ACTIVATION BY A P53-BINDING ELEMENT
- Authors:
- Published online on: November 1, 1995 https://doi.org/10.3892/ijo.7.5.1021
- Pages: 1021-1028
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Abstract
We conducted transient reporter gene expression assays to show that the wild-type but not the mutant-type p53 protein positively regulates the expression of the human H-ras gene. This activation of expression is mediated through a specific p53-binding DNA element located in the first intron of the H-ras gene. This element is similar to a previously identified p53-binding element. Without this element, wild-type p53 represses the H-ras promoter, as do several p53 mutants. The repression function is lost when the N-terminal 81 amino acids of the p53 protein are deleted, suggesting that the N-terminus is required for repression. Therefore, p53 seems to regulate the H-ras through both positive and negative mechanisms.
