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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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December 1995 Volume 7 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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December 1995 Volume 7 Issue 6

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Article

CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA

  • Authors:
    • JK SAGOO
    • C IAONNOU
    • NRA BEELEY
    • C SUTTON
    • CI DE-MATTEIS
    • SJB TENDLER

  • View Affiliations / Copyright

    Affiliations: UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,BIOPHYS & SURFACE ANAL LAB,NOTTINGHAM NG7 2RD,ENGLAND. CELLTECH LTD,SLOUGH SL1 4EN,BERKS,ENGLAND. FINNIGAN MAT CORP,HEMEL HEMPSTEAD HP2 4TG,HERTS,ENGLAND.
  • Pages: 1437-1443
    |
    Published online on: December 1, 1995
       https://doi.org/10.3892/ijo.7.6.1437
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Abstract

We report the characterization of a 14 kDa degradation fragment from recombinant human tumour necrosis factor-alpha (TNF alpha) by N-terminal sequencing and mass spectrometry. A single site between the dibasic residues Arg(31)-Arg(32) of the mature recombinant 17 kDa protein has been identified as the target site that generates the 14 kDa fragment. The observation that a maximum of 33% degradation occurs suggests that only one monomer per TNF trimer is cleaved. E. coli proteases specific for dibasic residues are thought to be responsible for this cleavage. A strategy has been developed which completely inhibits proteolysis. This strategy has been used to reduce the 14 kDa degradation fragment obtained from approximately 33% of the total purified protein to zero.

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Copy and paste a formatted citation
Spandidos Publications style
SAGOO J, IAONNOU C, BEELEY N, SUTTON C, DE-MATTEIS C and TENDLER S: CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA. Int J Oncol 7: 1437-1443, 1995.
APA
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DE-MATTEIS, C., & TENDLER, S. (1995). CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA. International Journal of Oncology, 7, 1437-1443. https://doi.org/10.3892/ijo.7.6.1437
MLA
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DE-MATTEIS, C., TENDLER, S."CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA". International Journal of Oncology 7.6 (1995): 1437-1443.
Chicago
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DE-MATTEIS, C., TENDLER, S."CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA". International Journal of Oncology 7, no. 6 (1995): 1437-1443. https://doi.org/10.3892/ijo.7.6.1437
Copy and paste a formatted citation
x
Spandidos Publications style
SAGOO J, IAONNOU C, BEELEY N, SUTTON C, DE-MATTEIS C and TENDLER S: CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA. Int J Oncol 7: 1437-1443, 1995.
APA
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DE-MATTEIS, C., & TENDLER, S. (1995). CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA. International Journal of Oncology, 7, 1437-1443. https://doi.org/10.3892/ijo.7.6.1437
MLA
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DE-MATTEIS, C., TENDLER, S."CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA". International Journal of Oncology 7.6 (1995): 1437-1443.
Chicago
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DE-MATTEIS, C., TENDLER, S."CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA". International Journal of Oncology 7, no. 6 (1995): 1437-1443. https://doi.org/10.3892/ijo.7.6.1437
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