A comparison of the detection of activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation analysis and enriched PCR in colorectal adenocarcinomas

  • Authors:
    • J Elnatan
    • H Goh
    • D Smith
  • View Affiliations

  • Published online on: January 1, 1996     https://doi.org/10.3892/ijo.8.1.139
  • Pages: 139-143
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Abstract

We have examined 56 colorectal adenocarcinomas for activation of the c-Ki-ras proto-oncogene by two independent techniques, namely mismatch specific oligonucleotide hybridisation and an enriched PCR methodology. Biological significance of c-Ki-ras activation was assessed via Kaplan Meier analysis of patient survival. A total of 27% (15/56) of tumours were found to contain activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation and mutation was significantly associated with a poorer patient prognosis (p=0.018, log rank analysis). A total of 55% (31/56) of tumours were found to contain activated c-Ki-ras genes by enriched PCR, and again an association was noted between mutation and patient survival, but of a lower significance (p=0.029, log rank analysis). These results indicate that a high proportion of colorectal adenocarcinomas may contain low levels of activated c-Ki-ras genes, but this may be of limited biological significance.

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January 1996
Volume 8 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Elnatan J, Goh H and Smith D: A comparison of the detection of activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation analysis and enriched PCR in colorectal adenocarcinomas. Int J Oncol 8: 139-143, 1996
APA
Elnatan, J., Goh, H., & Smith, D. (1996). A comparison of the detection of activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation analysis and enriched PCR in colorectal adenocarcinomas. International Journal of Oncology, 8, 139-143. https://doi.org/10.3892/ijo.8.1.139
MLA
Elnatan, J., Goh, H., Smith, D."A comparison of the detection of activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation analysis and enriched PCR in colorectal adenocarcinomas". International Journal of Oncology 8.1 (1996): 139-143.
Chicago
Elnatan, J., Goh, H., Smith, D."A comparison of the detection of activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation analysis and enriched PCR in colorectal adenocarcinomas". International Journal of Oncology 8, no. 1 (1996): 139-143. https://doi.org/10.3892/ijo.8.1.139