A comparison of the detection of activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation analysis and enriched PCR in colorectal adenocarcinomas
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- Published online on: January 1, 1996 https://doi.org/10.3892/ijo.8.1.139
- Pages: 139-143
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Abstract
We have examined 56 colorectal adenocarcinomas for activation of the c-Ki-ras proto-oncogene by two independent techniques, namely mismatch specific oligonucleotide hybridisation and an enriched PCR methodology. Biological significance of c-Ki-ras activation was assessed via Kaplan Meier analysis of patient survival. A total of 27% (15/56) of tumours were found to contain activated c-Ki-ras genes by mismatch specific oligonucleotide hybridisation and mutation was significantly associated with a poorer patient prognosis (p=0.018, log rank analysis). A total of 55% (31/56) of tumours were found to contain activated c-Ki-ras genes by enriched PCR, and again an association was noted between mutation and patient survival, but of a lower significance (p=0.029, log rank analysis). These results indicate that a high proportion of colorectal adenocarcinomas may contain low levels of activated c-Ki-ras genes, but this may be of limited biological significance.