Establishment of a continuous cell line from a human carcinoid of the small intestine (KRJ-I)

Pfragner,R; Wirnsberger,G; Niederle,B; Behmel,A; Rinner,I; Mandl,A; Wawrina,F; Luo,J; Adamiker,D; Hoger,H; Ingolic,E; Schauenstein,K

doi:10.3892/ijo.8.3.513

Establishment of a continuous cell line from a human carcinoid of the small intestine (KRJ-I)

Authors:
- R Pfragner
- G Wirnsberger
- B Niederle
- A Behmel
- I Rinner
- A Mandl
- F Wawrina
- J Luo
- D Adamiker
- H Hoger
- E Ingolic
- K Schauenstein
View Affiliations

Affiliations: GRAZ UNIV,SCH MED,DEPT INTERNAL MED,A-8010 GRAZ,AUSTRIA. GRAZ UNIV,SCH MED,DEPT MED BIOL & HUMAN GENET,A-8010 GRAZ,AUSTRIA. UNIV VIENNA,SCH MED,DEPT SURG,A-1010 VIENNA,AUSTRIA. UNIV VIENNA,RES INST LAB ANIM BREEDING,A-1010 VIENNA,AUSTRIA. RES INST ELECTRON MICROSCOPY & FINE STRUCT,GRAZ,AUSTRIA. GRAZ UNIV TECHNOL,A-8010 GRAZ,AUSTRIA.
Published online on: March 1, 1996 https://doi.org/10.3892/ijo.8.3.513
Pages: 513-520

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Abstract

A new continuous cell line from a human malignant carcinoid of the small intestine (KRJ-I) was established. The cells showed morphological and immunocytochemical features of the tumor of origin and expressed estrogen receptors. The cells are growing as a suspension, forming multicellular aggregates and spheroids. Electron microscopy confirmed the presence of neuroendocrine granules. Dose-dependent growth inhibition was observed after incubation with 5-azacytidine. Cytogenetic analyses of the tumor of origin, the cell line KRJ-I and a liver metastasis KRJ-II revealed clonal tetraploidy and clonal loss of the Y-chromosome and chromosome 19.