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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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April 1996 Volume 8 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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April 1996 Volume 8 Issue 4

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Article

Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells

  • Authors:
    • E Venturelli
    • D Coradini
    • D Gornati
    • G Secreto
  • View Affiliations / Copyright

    Affiliations: NCI,DIV NUCL MED,ENDOCRINE UNIT,I-20133 MILAN,ITALY. NCI,DIV EXPTL ONCOL C,I-20133 MILAN,ITALY.
  • Pages: 687-692
    |
    Published online on: April 1, 1996
       https://doi.org/10.3892/ijo.8.4.687
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Abstract

The purpose of our study was to evaluate the effect of testosterone and its metabolic pathway in MCF-7 cells in culture. Testosterone exhibited a dose-dependent (from 0.1 to 10 nM) and time-dependent (from 3 to 9 days) growth stimulation. The metabolic pathway was investigated following treatment with two testosterone concentrations: one stimulating (10 nM) and one not affecting (0.1 nM) cell growth. Celite column chromatography was used to separate H-3-testosterone metabolites, whose identity was confirmed by gas chromatography-mass spectrometry analysis. The main findings of the metabolic study were: i) recovery of a large amount of untransformed testosterone; ii) a high conversion of testosterone to conjugated, biologically inactive metabolites; iii) the highest level of Sa-diol among the metabolites of testosterone; iv) a conversion (2%) of testosterone into oestradiol, which resulted in a growth stimulatory concentration when testosterone was used at 10 nM. We conclude that in our experimental conditions androgens and oestrogens can concur to stimulate MCF-7 cell growth through androgen receptor-mediated and oestrogen receptor-mediated mechanisms.

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Copy and paste a formatted citation
Spandidos Publications style
Venturelli E, Coradini D, Gornati D and Secreto G: Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells. Int J Oncol 8: 687-692, 1996.
APA
Venturelli, E., Coradini, D., Gornati, D., & Secreto, G. (1996). Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells. International Journal of Oncology, 8, 687-692. https://doi.org/10.3892/ijo.8.4.687
MLA
Venturelli, E., Coradini, D., Gornati, D., Secreto, G."Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells". International Journal of Oncology 8.4 (1996): 687-692.
Chicago
Venturelli, E., Coradini, D., Gornati, D., Secreto, G."Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells". International Journal of Oncology 8, no. 4 (1996): 687-692. https://doi.org/10.3892/ijo.8.4.687
Copy and paste a formatted citation
x
Spandidos Publications style
Venturelli E, Coradini D, Gornati D and Secreto G: Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells. Int J Oncol 8: 687-692, 1996.
APA
Venturelli, E., Coradini, D., Gornati, D., & Secreto, G. (1996). Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells. International Journal of Oncology, 8, 687-692. https://doi.org/10.3892/ijo.8.4.687
MLA
Venturelli, E., Coradini, D., Gornati, D., Secreto, G."Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells". International Journal of Oncology 8.4 (1996): 687-692.
Chicago
Venturelli, E., Coradini, D., Gornati, D., Secreto, G."Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells". International Journal of Oncology 8, no. 4 (1996): 687-692. https://doi.org/10.3892/ijo.8.4.687
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