Anti-tumor ribonuclease, combined with or conjugated to monoclonal antibody MRK16, overcomes multidrug resistance to vincristine in vitro and in vivo

Newton,D; Pearson,J; Xue,Y; Smith,M; Fogler,W; Mikulski,S; Alvord,W; Kung,H; Long,D; Rybak,S

doi:10.3892/ijo.8.6.1095

Anti-tumor ribonuclease, combined with or conjugated to monoclonal antibody MRK16, overcomes multidrug resistance to vincristine in vitro and in vivo

Authors:
- D Newton
- J Pearson
- Y Xue
- M Smith
- W Fogler
- S Mikulski
- W Alvord
- H Kung
- D Long
- S Rybak
View Affiliations

Affiliations: NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,LAB BIOCHEM PHYSIOL,FREDERICK,MD 21702. SAIC,BCDP,FREDERICK,MD 21702. NCI,FREDERICK CANC RES & DEV CTR,BIOL RESPONSE MODIFIERS PROGRAM,LEI,FREDERICK,MD 21702. ALFACELL CORP,BLOOMFIELD,NJ 07003. NCI,FREDERICK CANC RES & DEV CTR,DATA MANAGEMENT SERV INC,FREDERICK,MD 21702.
Published online on: June 1, 1996 https://doi.org/10.3892/ijo.8.6.1095
Pages: 1095-1104

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Abstract

Onconase, a ribonuclease isolated from Rana pipiens oocytes and early embryos, is a member of the RNase A superfamily. Onconase has anti-neoplastic properties both in vitro and in vivo, and is undergoing clinical evaluation. In the present study, Onconase was combined with or conjugated to MRK16, an anti-P-glycoprotein (Pgp) monoclonal antibody. The interaction of these combinations with vincristine (VCR) against parental and multidrug resistant (MDR), Pgp expressing, human colon carcinoma cells caused increased VCR cytotoxicity in vitro and enhanced survival of athymic nude mice given transplants of drug resistant HT-29(mdr1) cells in vivo. The results suggest that combination treatment with Onconase and other agents that modulate the chemosensitivity of Pgp-expressing human tumor cells has the potential to overcome MDR.