Co-cultured endothelial and Kupffer cells regulate hepatocyte replication

  • Authors:
    • T Namieno
    • K Koito
    • N Sato
    • J Uchino
    • M Hosokawa
  • View Affiliations

  • Published online on: October 1, 1996     https://doi.org/10.3892/ijo.9.4.737
  • Pages: 737-740
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Abstract

We studied the effect of co-culture with endothelial and/or Kupffer cells on hepatocyte replication in primary culture by means of [H-3]-thymidine incorporation. Hepatocytes, sinusoidal endothelial and Kupffer cells were extracted from rat liver by a two-step perfusion and subsequent two-step elutriation method. The replication was promoted in proportion with the densities of endothelial cells, but inversely suppressed by co-culture with Kupffer cells. The replicative effect of endothelial cells was not observed with the high number of hepatocytes cultured, however. The hepatocyte replication was also regulated in accordance with the ratios of endothelial/Kupffer cells. These results indicate that endothelial cells have a positive effect and Kupffer cells have a negative effect on hepatocyte replication, and that this replication may be associated with hepatocarcinogenesis and/or tumor-growth of hepatocellular carcinoma.

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October 1996
Volume 9 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Namieno T, Koito K, Sato N, Uchino J and Hosokawa M: Co-cultured endothelial and Kupffer cells regulate hepatocyte replication. Int J Oncol 9: 737-740, 1996
APA
Namieno, T., Koito, K., Sato, N., Uchino, J., & Hosokawa, M. (1996). Co-cultured endothelial and Kupffer cells regulate hepatocyte replication. International Journal of Oncology, 9, 737-740. https://doi.org/10.3892/ijo.9.4.737
MLA
Namieno, T., Koito, K., Sato, N., Uchino, J., Hosokawa, M."Co-cultured endothelial and Kupffer cells regulate hepatocyte replication". International Journal of Oncology 9.4 (1996): 737-740.
Chicago
Namieno, T., Koito, K., Sato, N., Uchino, J., Hosokawa, M."Co-cultured endothelial and Kupffer cells regulate hepatocyte replication". International Journal of Oncology 9, no. 4 (1996): 737-740. https://doi.org/10.3892/ijo.9.4.737