Apoptotic activity of fatty acid derivatives may correlate with their inhibition of DNA replication

  • Authors:
    • Chunhui Miao
    • Juan Du
    • Hung The Dang
    • In-Hye Jeong
    • Song You
    • Jang-Su Park
    • Jee H. Jung
    • Dong-Kyoo Kim
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/ijo_00000120
  • Pages: 1291-1298
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Abstract

The apoptogenic and DNA damaging effects of (E)-10-oxooctadec-8-enoic acid (S5C) and (E)-9-oxooctadec-10-enoic acid (S6C), two structurally related fatty acids isolated from Red Alga Gracilaria verrucosa, were compared and their apoptosis-inducing properties characterized against human lung carcinoma (A549) cells. Significantly, the two acids decreased the rates of proliferation and viability (IC50 of ≈170 and ≈140 µM) as well as evidence of the induction of apoptosis. Cell morphological changes observed under light microscopy confirmed apoptosis occurrence. The results from Annexin V/PI dual staining and the cell cycle arrest assay indicated that S5C and S6C induced an earlier apoptosis of A549 cells in a concentration-dependent manner. We found that they induced DNA damage and inhibited DNA replication followed by S-phase arrest. In addition, the very sensitive alkaline micro-gel electrophoresis technique (comet assay) was used to estimate the compound-induced DNA single- and double-strand breaks. These findings suggest that S5C and S6C induced A549 cell apoptosis and their effects are associated with DNA damage. Therefore, S5C and S6C have the potential to be developed into anticancer agents due to their relatively easy synthesis and structural manipulation.

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December 2008
Volume 33 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Miao C, Du J, Dang HT, Jeong I, You S, Park J, Jung JH and Kim D: Apoptotic activity of fatty acid derivatives may correlate with their inhibition of DNA replication. Int J Oncol 33: 1291-1298, 2008
APA
Miao, C., Du, J., Dang, H.T., Jeong, I., You, S., Park, J. ... Kim, D. (2008). Apoptotic activity of fatty acid derivatives may correlate with their inhibition of DNA replication. International Journal of Oncology, 33, 1291-1298. https://doi.org/10.3892/ijo_00000120
MLA
Miao, C., Du, J., Dang, H. T., Jeong, I., You, S., Park, J., Jung, J. H., Kim, D."Apoptotic activity of fatty acid derivatives may correlate with their inhibition of DNA replication". International Journal of Oncology 33.6 (2008): 1291-1298.
Chicago
Miao, C., Du, J., Dang, H. T., Jeong, I., You, S., Park, J., Jung, J. H., Kim, D."Apoptotic activity of fatty acid derivatives may correlate with their inhibition of DNA replication". International Journal of Oncology 33, no. 6 (2008): 1291-1298. https://doi.org/10.3892/ijo_00000120