Cellular plasticity of trans- and dedifferentiation markers in human hepatoma cells in vitro and in vivo

  • Authors:
    • Samir Jabari
    • Matthias Meissnitzer
    • Karl Quint
    • Susanne Gahr
    • Till Wissniowski
    • Eckhart G. Hahn
    • Daniel Neureiter
    • Matthias Ocker
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/ijo_00000314
  • Pages: 69-80
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Abstract

Tumor cells have the capability to trans- and to dedifferentiate, for example by reactivating embryonic development genes and stem cell characteristics. The aim of our study was to show the differential expression of stem- and progenitor cell markers in human hepatocellular carcinoma cell lines (HCC). Different human HCC cell lines (HUH7, HUH7 5-15, HUH7 pcDNA3.1, Hep3B and HepG2) were cultured under standard conditions in vitro or implanted subcutaneously (5x106 cells) in male NMRI mice. Specimens were characterized by quantitative real-time PCR, Western blotting, methylation-specific PCR and immunohistochemistry for markers of differentiation (cytokeratins, vimentin), embryonic development or stem cells (PTC, PDX-1, SHH, Thy1, c-kit, CD34, β-catenin, Ki-67). The investigated HCC cell lines showed different patterns of marker expression allowing to distinguish four distinct groups: the classical cholangiocellular type (Huh-7, Huh-7 pcDNA3.1, Hep3B) with expression of CK7/19, β-catenin and CD34; a dedifferentiated mesenchymal-proliferative type (Huh-7 5-15) characterized by CK19, Vimentin and Ki-67; a dedifferentiated embryonic-development type (Hep3B implanted in matrigel) with expression of CK19, β-catenin and PTC and a classical HCC type (HepG2) showing CK18/19 and β-catenin expression. HCC cell lines showed significantly different expression patterns of differentiation markers in a xenograft model. Furthermore, direct association of some markers was observed. The groups differ from each other in expression patterns, but also show that environmental factors play an important role in the behaviour of cells.

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July 2009
Volume 35 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Jabari S, Meissnitzer M, Quint K, Gahr S, Wissniowski T, Hahn EG, Neureiter D and Ocker M: Cellular plasticity of trans- and dedifferentiation markers in human hepatoma cells in vitro and in vivo. Int J Oncol 35: 69-80, 2009.
APA
Jabari, S., Meissnitzer, M., Quint, K., Gahr, S., Wissniowski, T., Hahn, E.G. ... Ocker, M. (2009). Cellular plasticity of trans- and dedifferentiation markers in human hepatoma cells in vitro and in vivo. International Journal of Oncology, 35, 69-80. https://doi.org/10.3892/ijo_00000314
MLA
Jabari, S., Meissnitzer, M., Quint, K., Gahr, S., Wissniowski, T., Hahn, E. G., Neureiter, D., Ocker, M."Cellular plasticity of trans- and dedifferentiation markers in human hepatoma cells in vitro and in vivo". International Journal of Oncology 35.1 (2009): 69-80.
Chicago
Jabari, S., Meissnitzer, M., Quint, K., Gahr, S., Wissniowski, T., Hahn, E. G., Neureiter, D., Ocker, M."Cellular plasticity of trans- and dedifferentiation markers in human hepatoma cells in vitro and in vivo". International Journal of Oncology 35, no. 1 (2009): 69-80. https://doi.org/10.3892/ijo_00000314