hTERT promotes the invasion of telomerase-negative tumor cells in vitro

  • Authors:
    • Song-Tao Yu
    • Ling Chen
    • Hong-Jin Wang
    • Xu-Dong Tang
    • Dian-Chun Fang
    • Shi-Ming Yang
  • View Affiliations

  • Published online on: August 1, 2009     https://doi.org/10.3892/ijo_00000344
  • Pages: 329-336
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Telomerase activation is a common feature of most types of human cancers. Although several studies have shown that activation of telomerase might participate in the progression of tumors, the mechanism by which telomerase activation causes the invasion and metastasis of tumors remains unclear. In this study, we transfected a vector containing the full-length cDNA of hTERT into a telomerase-negative osteosarcoma cell line U2OS (hTERT/U2OS). Vacant vector-transfected U2OS cells served as a control (EGFP/U2OS). We then compared the biological and vitodynamic changes in these transfected and untransfected U2OS cells. The hTERT protein was detected in hTERT/U2OS cells by Western blot analysis and immunochemistry assay. The telomere length in hTERT/U2OS cells was longer than that in EGFP/U2OS and untransfected U2OS cells. We also found using vacuum micropipette aspiration that hTERT transfection did not only promote the proliferation of hTERT-transfected U2OS cells but also increased the cellular adhesion capacity to the extracellular matrix. Transwell matrigel assay confirmed an increased invasion ability in hTERT/U2OS cells. These results strongly suggest that hTERT transfection promotes the invasion of telomerase-negative cells. Telomerase-mediated telomere maintenance enables these cells to achieve a fully malignant endpoint, including invasion and metastasis.

Related Articles

Journal Cover

August 2009
Volume 35 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yu S, Chen L, Wang H, Tang X, Fang D and Yang S: hTERT promotes the invasion of telomerase-negative tumor cells in vitro. Int J Oncol 35: 329-336, 2009.
APA
Yu, S., Chen, L., Wang, H., Tang, X., Fang, D., & Yang, S. (2009). hTERT promotes the invasion of telomerase-negative tumor cells in vitro. International Journal of Oncology, 35, 329-336. https://doi.org/10.3892/ijo_00000344
MLA
Yu, S., Chen, L., Wang, H., Tang, X., Fang, D., Yang, S."hTERT promotes the invasion of telomerase-negative tumor cells in vitro". International Journal of Oncology 35.2 (2009): 329-336.
Chicago
Yu, S., Chen, L., Wang, H., Tang, X., Fang, D., Yang, S."hTERT promotes the invasion of telomerase-negative tumor cells in vitro". International Journal of Oncology 35, no. 2 (2009): 329-336. https://doi.org/10.3892/ijo_00000344