Down-regulation of the apoptosis-inducing factor or Bcl-2 inhibitor of transcription by RNA interference can alleviate TAp63γ-induced apoptosis in esophageal squamous carcinoma EC9706 cells

  • Authors:
    • Tianli Fan
    • Guozhong Jiang
    • Zhenhe Suo
    • Hongtao Liu
    • Peirong Xu
    • Zhenyu Ji
    • Lirong Zhang
    • Guanrui Yang
  • View Affiliations

  • Published online on: August 1, 2009     https://doi.org/10.3892/ijo_00000347
  • Pages: 359-367
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Abstract

In this communication, the roles of apoptosis-inducing factor (AIF) and Bcl-2 inhibitor of transcription (Bit1) in TAp63γ-induced apoptosis were investigated in human esophageal squamous cancer EC9706 cells. Positive RNA and protein expressions of AIF and Bit1 in these cells were verified. However, no TAp63γ could be detected. Transfection of expression vector pcDNA3.1-TAp63γ into EC9706 resulted in TAp63γ expression, and peak level apoptosis was observed 24 h after the transfection disclosed by DNA fragmentation assay. In addition, it was found with Western blot that AIF and Bit1 were released into cytosol from mitochondria, and AIF was further translocated into nucleus, during the stage of TAp63γ-induced cell apoptosis. Down-regulation of either AIF or Bit1 by RNA interference could, however, alleviate TAp63γ-induced cell apoptosis. In conclusion, TAp63γ could induce apoptosis in human esophageal squamous cancer EC9706 cells, through at least releasing AIF and Bit1 from mitochindria into cytosol and nucleus, where apoptotic cascade takes place. These findings indicate that mitochondria-released proapoptotic proteins, AIF and Bit1, are important factors in a TAp63γ-induced EC9706 cell apoptosis pathway.

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August 2009
Volume 35 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Fan T, Jiang G, Suo Z, Liu H, Xu P, Ji Z, Zhang L and Yang G: Down-regulation of the apoptosis-inducing factor or Bcl-2 inhibitor of transcription by RNA interference can alleviate TAp63γ-induced apoptosis in esophageal squamous carcinoma EC9706 cells. Int J Oncol 35: 359-367, 2009.
APA
Fan, T., Jiang, G., Suo, Z., Liu, H., Xu, P., Ji, Z. ... Yang, G. (2009). Down-regulation of the apoptosis-inducing factor or Bcl-2 inhibitor of transcription by RNA interference can alleviate TAp63γ-induced apoptosis in esophageal squamous carcinoma EC9706 cells. International Journal of Oncology, 35, 359-367. https://doi.org/10.3892/ijo_00000347
MLA
Fan, T., Jiang, G., Suo, Z., Liu, H., Xu, P., Ji, Z., Zhang, L., Yang, G."Down-regulation of the apoptosis-inducing factor or Bcl-2 inhibitor of transcription by RNA interference can alleviate TAp63γ-induced apoptosis in esophageal squamous carcinoma EC9706 cells". International Journal of Oncology 35.2 (2009): 359-367.
Chicago
Fan, T., Jiang, G., Suo, Z., Liu, H., Xu, P., Ji, Z., Zhang, L., Yang, G."Down-regulation of the apoptosis-inducing factor or Bcl-2 inhibitor of transcription by RNA interference can alleviate TAp63γ-induced apoptosis in esophageal squamous carcinoma EC9706 cells". International Journal of Oncology 35, no. 2 (2009): 359-367. https://doi.org/10.3892/ijo_00000347