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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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August 2009 Volume 35 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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August 2009 Volume 35 Issue 2

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Article

Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing

  • Authors:
    • Susan Sewart
    • Roger Barraclough
    • Philip S. Rudland
    • Christopher R. West
    • Dong Liu Barraclough

  • View Affiliations / Copyright

    Affiliations: Cancer Tissue Bank Research Centre, University of Liverpool, Liverpool L69 7ZB, UK
  • Pages: 381-386
    |
    Published online on: August 1, 2009
       https://doi.org/10.3892/ijo_00000350
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Abstract

It is critical for both basic and clinical translational cancer research to use high quality DNA, RNA and proteins from specimens with clinical outcome in order to validate novel diagnostic biomarkers and to monitor successful treatments for patients. However, using current standard procedures, the collection of specimens is often limited by the availability of liquid nitrogen in some hospitals and liquid nitrogen can be hazardous to transport. These problems would be eased if the tissue could be stored unfixed at 4°C, conditions that are readily available in hospitals. Thus the effect of storing tissue specimens at 4°C on the quality of DNA, RNA and protein has been examined. Clinical tissue samples were halved and kept either at 4°C for up to 24 h or snap-frozen in liquid nitrogen within 30 min of removal from the patient. The results show that the quality of RNA, DNA and protein isolated from the specimens stored at 4°C up to overnight is equal to that obtained from snap-frozen material. In conclusion, simplifying the collection procedure may allow for greater flexibility of conducting studies in units where liquid nitrogen is not readily available.

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Copy and paste a formatted citation
Spandidos Publications style
Sewart S, Barraclough R, Rudland PS, West CR and Barraclough DL: Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing. Int J Oncol 35: 381-386, 2009.
APA
Sewart, S., Barraclough, R., Rudland, P.S., West, C.R., & Barraclough, D.L. (2009). Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing. International Journal of Oncology, 35, 381-386. https://doi.org/10.3892/ijo_00000350
MLA
Sewart, S., Barraclough, R., Rudland, P. S., West, C. R., Barraclough, D. L."Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing". International Journal of Oncology 35.2 (2009): 381-386.
Chicago
Sewart, S., Barraclough, R., Rudland, P. S., West, C. R., Barraclough, D. L."Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing". International Journal of Oncology 35, no. 2 (2009): 381-386. https://doi.org/10.3892/ijo_00000350
Copy and paste a formatted citation
x
Spandidos Publications style
Sewart S, Barraclough R, Rudland PS, West CR and Barraclough DL: Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing. Int J Oncol 35: 381-386, 2009.
APA
Sewart, S., Barraclough, R., Rudland, P.S., West, C.R., & Barraclough, D.L. (2009). Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing. International Journal of Oncology, 35, 381-386. https://doi.org/10.3892/ijo_00000350
MLA
Sewart, S., Barraclough, R., Rudland, P. S., West, C. R., Barraclough, D. L."Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing". International Journal of Oncology 35.2 (2009): 381-386.
Chicago
Sewart, S., Barraclough, R., Rudland, P. S., West, C. R., Barraclough, D. L."Molecular analysis of a collection of clinical specimens stored at 4°C as an alternative to snap-freezing". International Journal of Oncology 35, no. 2 (2009): 381-386. https://doi.org/10.3892/ijo_00000350
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