Melphalan inhibits adenoma development through modulating the expression of K-ras-specific markers in K-ras Tg mice

  • Authors:
    • Sojung Lee
    • Heesook Choi
    • Eunjin Kim
    • Heejong Kim
    • Young-Ho Park
    • Dae-Yeul Yu
    • Seok-Joo Yoon
    • Jinman Kim
    • Yhunyhong Sheen
    • Sue-Nie Park
    • Do-Young Yoon
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ijo_00000670
  • Pages: 219-228
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Abstract

In previous research, we focused on the discovery of K-ras biomarkers, and effects of genotoxic carcinogens on their expression were investigated in this study. It is well-known that mutated K-ras gene is involved in ≈30% of human cancers such as lung cancer. To search for K-ras oncogene-induced modulators in lung tissues of K-ras transgenic mice, we analyzed K-ras-specific genes and proteins related to cancer development, signal transduction, inflammation as well as tumor suppression in a previous study. In this study, we investigated the modulating effects of genotoxic carcinogen treatment on expression of K-ras-dependent modulated genes and proteins in lung tissues of K-ras Tg mice. In order to evaluate candidate K-ras markers modulated by genotoxic stress and to investigate whether a genotoxic carcinogen would enhance or inhibit carcinogenesis in lung tissues of the K-ras Tg mice, the anti-cancer drug melphalan was intraperitoneally injected into K-ras Tg mice every two days for four weeks. RT-qPCR and proteomics analyses were performed in order to confirm whether K-ras-specific biomarkers would be modulated by melphalan treatment in K-ras Tg mice. The decreased adenomas were histopathologically observed and K-ras expression was suppressed in melphalan-treated K-ras Tg mice. Melphalan also recovered the expression of K-ras-dependent modulated biomarkers. These results suggest that melphalan inhibits carcinogenesis via modulating K-ras-specific genes and proteins expressed in the lung tissues of K-ras Tg mice.

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July 2010
Volume 37 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lee S, Choi H, Kim E, Kim H, Park Y, Yu D, Yoon S, Kim J, Sheen Y, Park S, Park S, et al: Melphalan inhibits adenoma development through modulating the expression of K-ras-specific markers in K-ras Tg mice. Int J Oncol 37: 219-228, 2010
APA
Lee, S., Choi, H., Kim, E., Kim, H., Park, Y., Yu, D. ... Yoon, D. (2010). Melphalan inhibits adenoma development through modulating the expression of K-ras-specific markers in K-ras Tg mice. International Journal of Oncology, 37, 219-228. https://doi.org/10.3892/ijo_00000670
MLA
Lee, S., Choi, H., Kim, E., Kim, H., Park, Y., Yu, D., Yoon, S., Kim, J., Sheen, Y., Park, S., Yoon, D."Melphalan inhibits adenoma development through modulating the expression of K-ras-specific markers in K-ras Tg mice". International Journal of Oncology 37.1 (2010): 219-228.
Chicago
Lee, S., Choi, H., Kim, E., Kim, H., Park, Y., Yu, D., Yoon, S., Kim, J., Sheen, Y., Park, S., Yoon, D."Melphalan inhibits adenoma development through modulating the expression of K-ras-specific markers in K-ras Tg mice". International Journal of Oncology 37, no. 1 (2010): 219-228. https://doi.org/10.3892/ijo_00000670