Expression of ADAMTS4 in Ewing's sarcoma

Minobe,K.; Ono,R.; Matsumine,A.; Shibata-Minoshima,F.; Izawa,K.; Oki,T.; Kitaura,J.; Iino,T.; Takita,J.; Iwamoto,S.; Hori,H.; Komada,Y.; Uchida,A.; Hayashi,Y.; Kitamura,T.; Nosaka,T.

doi:10.3892/ijo_00000706

September 2010 Volume 37 Issue 3

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September 2010 Volume 37 Issue 3

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Article

Expression of ADAMTS4 in Ewing's sarcoma

Authors:
- K. Minobe
- R. Ono
- A. Matsumine
- F. Shibata-Minoshima
- K. Izawa
- T. Oki
- J. Kitaura
- T. Iino
- J. Takita
- S. Iwamoto
- H. Hori
- Y. Komada
- A. Uchida
- Y. Hayashi
- T. Kitamura
- T. Nosaka
View Affiliations / Copyright

Affiliations: Department of Microbiology and Molecular Genetics, Mie University Graduate School of Medicine, Tsu 514-8507, Japan
Pages: 569-581
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Published online on: September 1, 2010

https://doi.org/10.3892/ijo_00000706
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Abstract

Ewing's sarcoma (EWS) is a malignant bone tumor that frequently occurs in teenagers. Genetic mutations which cause EWS have been investigated, and the most frequent one proved to be a fusion gene between EWS gene of chromosome 22 and the FLI1 gene of chromosome 11. However, a limited numbers of useful biological markers for diagnosis of EWS are available. In this study, we identified ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs) as a possible tumor marker for EWS using the retrovirus-mediated signal sequence trap method. ADAMTS4 is a secreted protein of 837 amino acids with a predicted molecular mass of 98-100 kDa. It is a member of metalloprotease family, is expressed mainly in cartilage and brain, and regulates the degradation of aggrecans. ADAMTS4 has been suggested to be involved in arthritic diseases and gliomas. Herein, we show that ADAMTS4 mRNA was expressed in all primary EWS samples and all EWS-derived cell lines examined, while its expression was detected only in small subpopulations of other solid tumors. Furthermore, ADAMTS4 expression was found to be regulated by EWS-FLI1 fusion gene-dependent manner. We also demonstrated that ADAMTS4 protein was highly expressed in tumor samples of the patients with EWS by using immunohistochemistry. These results suggest that ADAMTS4 is a novel tumor marker for EWS.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression of ADAMTS4 in Ewing's sarcoma

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Abstract

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