Open Access

Guillain‑Barre syndrome induced by pembrolizumab and sunitinib: A case report

  • Authors:
    • Chen Han
    • Jin‑An Ma
    • Ying Zhang
    • Yuna Jiang
    • Chunhong Hu
    • Yuanqiang Wu
  • View Affiliations

  • Published online on: May 5, 2020     https://doi.org/10.3892/mco.2020.2042
  • Pages: 38-42
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pembrolizumab, an immune checkpoint inhibitor against the programmed death‑1 pathway, has been used in combination with acitinib for the first‑line treatment of advanced renal cell carcinoma. Neurotoxicity is a rare immune‑related adverse event (irAE). The present study reports a case of Guillain‑Barre syndrome (GBS) induced by pembrolizumab and sunitinib, and reviews other previous studies to elucidate the clinical characteristics and suitable management of this rare irAE. An advanced renal cell carcinoma patient who received several cycles of pembrolizumab combined with sunitinib developed limb weakness and numbness of the extremities, and was diagnosed with GBS by electrodiagnostic and cerebrospinal fluid examination. The patient improved after treatment with intravenous immunoglobulin along with prednisone. To the best of our knowledge, this is the first case of GBS during treatment with pembrolizumab in combination with sunitinib in advanced renal cell carcinoma.

Introduction

Programmed death-1 (PD-1) inhibitors, including pembrolizumab and nivolumab, can activate T cells to kill tumor cells by blocking the binding of the PD-1 receptor and programmed death ligand 1 and 2 (PD-L1 and PD-L2) (1). It is important to note that the dramatic efficacies of PD-1 inhibitors commonly accompanied by fatal side effects such as cardiotoxicity, pneumonitis and neurological toxicities (2). Guillain-Barre syndrome (GBS) is a rare but fatal autoimmune-mediated peripheral neuropathy characterized by muscular weakness in the extremities and peripheral paresthesia. The pathogenesis of GBS is the autoimmune response caused by molecular mimicry between microbial and nerve antigens (3). The present study reports on the case of a patient with advanced renal cell carcinoma who received pembrolizumab combined with sunitinib and presented with GBS. To date, no similar case has been reported. We reviewed some literatures to analyze the underlying mechanism of this rare disease and we aim to highlight that early recognition and suitable management is crucial to improve patient outcomes.

Case report

A 55-year-old male presented with a right renal mass on CT scan. He was diagnosed with renal clear cell carcinoma with sarcomatoid differentiation after robot-assisted laparoscopic resection of the local mass (Fig. 1). Adjuvant targeted therapy with sunitinib (50 mg/day; 2 weeks on, 1 week off) was performed for six cycles. On March 21st 2018, a CT scan revealed local recurrence, and spleen and abdominal cavity metastasis (Fig. 2). As a result of this, the patient was started on pembrolizumab (2 mg/kg once every 3 weeks) combined with sunitinib (37.5 mg/day; 2 weeks on, 1 week off) on March 27th 2018. After 4 months, the patient developed limb weakness and numbness of the extremities. Neurological examination showed paresthesia of the four extremities, absence of tendon reflex of the limbs, and a mild decrease in muscle strength of the limbs with a grade of 4/5, but no pathological reflex was observed. This patient had no history of GBS, and laboratory examination results showed no evidence of infection. Procalcitonin, c-reactive protein, influenza virus antibody, cytomegalovirus antibody, rubella virus antibody, herpes simplex virus antibody, Epstein-Barr virus antibody, detection of mycoplasma and chlamydia DNA, and hepatitis virus tests were all negative. Rheumatic immune disease was also ruled out. Magnetic resonance imaging of the brain and spinal cord was normal. Cerebrospinal fluid (CSF) analysis showed albuminocytologic dissociation (protein, 583.30 mg/l; cell count, 8,000/µl), and Pandy's test was positive. Electroneuromyography revealed acute motor and sensory axonal neuropathy (Fig. 3). Based on these results, the patient was diagnosed with GBS, which can be induced by pembrolizumab. Pembrolizumab was permanently discontinued due to this severe immune-related adverse event (irAE). The symptoms were gradually aggravated after oral treatment with dexamethasone (10 mg/day). The patient was then switched to intravenous immunoglobulin (IVIG; 0.4 g/kg for 5 days) along with prednisone (1 mg/kg per day). The patient's symptoms improved during 1 week of treatment. After 1 month, although he experienced difficulty with fine movements, he was able to walk and stand normally, and the doctors began to taper off the prednisone dosage. CT scan reexamination showed that the masses in the right kidney and abdominal cavity had disappeared, and the enhancement degree of spleen metastases was reduced (Fig. 4). No recurrence was observed during the 10 months of follow-up.

Discussion

Pembrolizumab is a potent and highly selective fully human IgG4 PD-1 immune-checkpoint inhibitor. Studies have confirmed its efficacy in several types of advanced cancer, such as melanoma, non-small cell lung cancer, head and neck carcinoma and renal cancer (3-6). Immunotherapy can cause a series of irAEs, including skin reactions, colitis, hepatitis, endocrinopathies, pneumonitis and, rarely, neurological toxicity (7,8).

GBS is an autoimmune-mediated disease in which most patients have prodromal infection. Common infectious pathogens include cytomegalovirus, Epstein-Barr virus, influenza virus, human immunodeficiency virus, mycoplasma, Haemophilus and Campylobacter jejuni (1). It is now believed that the primary pathogenesis is the autoimmune response caused by molecular mimicry between microbial and nerve antigens. Both cellular and humoral immunity are abnormally activated. T cells invade the peripheral nerves, and peripheral demyelination is mediated by macrophages. Immunoglobulin and complement deposits appear on the myelin sheath and Schwann cells (9). Regulatory T cells (Treg cells) can downregulate the immune response, maintain autoimmunological tolerance, and prevent autoimmune diseases (10). The binding of PD-1 and its ligands PD-L1/PD-L2 causes CD25+Foxp3+ Treg cells to suppress anti-tumor immunity (11), and an increase in the number of CD25+Foxp3+ Treg cells has been reported in many malignant tumors (12-15), which is often associated with poor prognosis (16). The effect of PD-1 inhibitors is not limited to tumor-specific T cells, and blocking PD-1/PD-L1 and PD-L2 signals not only promotes anti-tumor immunity, but it also inhibits the generation of Treg cells in normal tissues, causing autoimmune adverse events (11). The patient in the present study had no symptoms of preexisting infection, and influenza virus, Epstein-Barr virus, human immunodeficiency virus, mycoplasma, hepatitis virus and cytomegalovirus infection were excluded by laboratory tests. It has been suggested that pembrolizumab may cause immune hyperfunction by increasing T cell activity, promoting T cell proliferation and inhibiting Treg cell function, thus disrupting immune homeostasis and inducing GBS.

Sunitinib is a multi-target tyrosine kinase receptor inhibitor targeting vascular endothelial growth factor receptor (VEGFR)-1, VEGFR-2, VEGFR-3, platelet-derived growth factor-α receptor (PDGFR-α), PDGFR-β, stem cell receptor and colony-stimulating factor 1 receptor, which was approved by the US Food and Drug Administration for the first-line treatment of metastatic renal cell carcinoma, and postoperative adjuvant treatment of renal cell carcinoma with a high risk of recurrence (17,18). In the KEYNOTE-426 study, pembrolizumab in combination with acitinib for advanced renal cell carcinoma significantly improved median progression-free survival (15.1 months vs. 11.1 months) over sunitinib alone (6), suggesting that pembrolizumab combined with a multi-target tyrosine kinase receptor inhibitor may be a promising treatment option. The most common side effects of sunitinib include fatigue, anorexia, hypertension, myelosuppression, diarrhea, mucositis, rashes and hand-foot syndrome (19). GBS developed in the patient in the present study, who had favorable responses to sunitinib in combination with pembrolizumab. Currently, two cases of GBS induced by sunitinib have been reported (20,21), to the best of our knowledge, and the mechanism may be related to the sunitinib-mediated inhibition of VEGFRs causing a corresponding increase in VEGF levels, which increases the numbers of B lymphocytes and immature myeloid cells (22). Elevated VEGF levels may also disrupt the blood-nerve barrier by altering microvascular permeability (22). Considering that both T cells and B cells are important in the pathogenesis of GBS, it is speculated that combination therapy may have a synergistic pathogenicity. However, further studies are warranted to confirm this hypothesis.

Management of irAEs must never be disregarded. In the PubMed database, eight cases of nivolumab and four cases of pembrolizumab causing GBS have been reported (23-33) (Table I). Many of these cases were treated with nivolumab or pembrolizumab monotherapy (10/13); two patients were treated with ipilimumab and nivolumab in combination, and one was treated with pembrolizumab followed by sequential dabrafenib and trametinib. Most cases were male (9/12), and the neurological symptoms were mostly sensory and movement disorders, and reduced or absent deep tendon reflexes and only one patient had a precursor infection. CSF tests showed that a high proportion of patients had albuminocytologic dissociation (9/12), and the etiological examinations were all negative. Most patients recovered with corticosteroids, IVIG and plasmapheresis, but two died of respiratory failure. The present patient did not improve with corticosteroids alone, but IVIG was effective, suggesting that IVIG may be suitable for checkpoint inhibitor-induced peripheral neuropathy, although corticosteroids are recommended for the management of irAEs. To the best of our knowledge, this is the first case of GBS during treatment with pembrolizumab in combination with sunitinib in advanced renal cell carcinoma, highlighting the importance of correctly identifying immune-related neurotoxicity and accumulating experience in the management of GBS induced by pembrolizumab and sunitinib.

Table I

Literature regarding GBS induced by PD-1 inhibitors.

Table I

Literature regarding GBS induced by PD-1 inhibitors.

StudyDisease typeCheckpoint inhibitorsGBS treatmentOutcomeCSF(Refs.)
Fukumoto et al, 2018NSCLCNivolumabIVIG, steroidsAliveAlbuminocytologic dissociation(23)
Gu et al, 2017MelanomaNivolumab+ ipilimumabIVIG, steroids, plasma exchange, mycophenolateAliveAlbuminocytologic dissociation(24)
Jacob et al, 2016NSCLCNivolumabIVIG, plasma exchangeDeadAlbuminocytologic dissociation(25)
Kyriazoglou et al, 2019Bladder cancerNivolumabIVIG, steroidsAliveAlbuminocytologic dissociation(26)
Nukui et al, 2018Nasal cancerNivolumabIVIG, steroidsAliveAlbuminocytologic dissociation(27)
Schneiderbauer et al, 2017MelanomaNivolumabIVIG, steroidsAliveAlbuminocytologic dissociation(28)
Supakornnumporn et al, 2017MelanomaNivolumab+ ipilimumabIVIG, steroidsAliveAlbuminocytologic dissociation(29)
Thapa et al, 2018NSCLCNivolumabIVIG, steroidsAliveNormal(30)
Manam et al, 2018NSCLCPembrolizumabIVIG, steroids, plasma exchangeAliveAlbuminocytologic dissociation(32)
Manam et al, 2018Melanoma Pembrolizumab-dabrafenib and trametinibIVIG, steroids, plasma exchangeDeadAlbuminocytologic dissociation(32)
de Maleissye et al, 2016MelanomaPembrolizumabIVIG, steroidsAliveAlbuminocytologic dissociation(31)
Ong et al, 2018NSCLCPembrolizumabIVIG, steroidsAliveUnknown(33)

[i] PD-1, programmed death-1; GBS, Guillain-Barre syndrome; CSF, cerebrospinal fluid; NSCLC, non-small cell lung cancer; IVIG, intravenous immunoglobulin.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availability of data and materials

All data generated or analyzed during the present study are included in this published article.

Authors' contributions

CHa designed the experiments and wrote the initial draft of the manuscript. JM, YZ, YJ, CHu and YW performed the experiments and assisted in the preparation of the manuscript. JM and YZ analyzed the data. CHa, CHu and YW revised the manuscript. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

The reported case has been approved by the patient for academic exchange only.

Competing interests

The authors declare that they have no competing interests.

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Spandidos Publications style
Han C, Ma JA, Zhang Y, Jiang Y, Hu C and Wu Y: Guillain‑Barre syndrome induced by pembrolizumab and sunitinib: A case report. Mol Clin Oncol 13: 38-42, 2020
APA
Han, C., Ma, J., Zhang, Y., Jiang, Y., Hu, C., & Wu, Y. (2020). Guillain‑Barre syndrome induced by pembrolizumab and sunitinib: A case report. Molecular and Clinical Oncology, 13, 38-42. https://doi.org/10.3892/mco.2020.2042
MLA
Han, C., Ma, J., Zhang, Y., Jiang, Y., Hu, C., Wu, Y."Guillain‑Barre syndrome induced by pembrolizumab and sunitinib: A case report". Molecular and Clinical Oncology 13.1 (2020): 38-42.
Chicago
Han, C., Ma, J., Zhang, Y., Jiang, Y., Hu, C., Wu, Y."Guillain‑Barre syndrome induced by pembrolizumab and sunitinib: A case report". Molecular and Clinical Oncology 13, no. 1 (2020): 38-42. https://doi.org/10.3892/mco.2020.2042