A rare intrahepatic splenosis mimicking hepatocellular carcinoma: A case report

Introduction

Splenosis is a rare benign condition occurring after trauma, splenectomy, or other procedures involving splenic tissue. It occurs when splenic sinus cells are transplanted directly into different compartments of the abdominal cavity or into the thorax (1). Distant transplantation of splenic tissue can occur by hematogenous spread to different organs, such as the liver, skin, or brain (2). Intrahepatic splenosis (IHS) is a condition in which splenic tissue is embedded within the liver parenchyma, and it was first described in the literature in 1939(3). It is usually detected incidentally during a physical examination or on imaging, some cases presented with diarrhea, pain, or bowel obstruction. The average interval between the initial trauma and detection is 16 years (range: 5 months to 32 years) (4), and cases of up to 60 years interval have been reported (5). Radiological findings are usually non-specific (6-10). Typical findings are hypodense areas on abdominal ultrasonography and non-contrast CT. Following contrast administration, IHS is hyperdense in the arterial phase, and hypodense in the delayed phase (3). On MRI, IHS is described as areas of homogeneous hypo-intensity in T1WI and hyperintensity in T2WI (10). Clinically, it is sometimes preoperatively diagnosed as a malignant tumor and surgical treatment should be inevitable. We report our experience with a rare case of IHS in which the imaging pattern was suggestive of hepatocellular carcinoma (HCC).

Case report

A 46-year-old man with a previous treatment history of a left lateral segmentectomy of the liver and splenectomy for a road traffic injury 30 years earlier presented to our emergency department with acute abdominal pain. The patient had no weight loss and no history of hepatitis or heavy drinking. The routine blood tests, including liver and renal function, were normal, and the serological biomarkers of hepatitis B and C virus infections were negative. Serum tumor marker levels for alpha-fetoprotein, CEA, CA19-9, and CA-125 were normal.

Abdominal ultrasonography (US) revealed a 2.4x1.4 cm isoechoic lesion with a hypoechoic zone at the margins in segment 7 of the right lobe of the liver (Fig. 1A). Abdominal plain-computed tomography (CT) revealed a hypodense mass measuring 2.5x1.7 cm (Fig. 1B). Abdominal magnetic resonance imaging (MRI) revealed a homogeneously hypointense mass in T1-weighted images and hyperintensity in T2-weighted images (Fig. 2A and B). The mass showed a heterogeneous hyperintensity in diffusion-weighted images and signal reduction in apparent diffusion coefficient. (Fig. 2C and D). After injecting gadoxetic acid, the lesion appeared strongly heterogeneous and hyperintense during the early phase and relatively hypointense during delayed phase that is ‘washout pattern’ (Fig. 2E and F). An indication of a pseudocapsule was also seen.

Figure 1 Abdominal ultrasonography and abdominal plain-computed tomography imaging of the IHS. (A) An abdominal ultrasonography revealed a 2.4x1.4 cm isoechoic lesion (arrows) with a hypoechoic zone at the margins in segment 7 of the liver. (B) An abdominal plane-computed tomography scan revealed a hypodense mass (arrows) measuring 2.5x1.7 cm in segment 7 of the liver.

Figure 2 Magnetic resonance imaging of the IHS (arrows). The IHS was detected with (A) a homogeneous hypointensity in T1-weighted images, (B) slight hyperintensity in T2-weighted images. (C) Lesion showed a heterogeneous hyperintensity in diffusion-weighted images and (D) signal reduction in apparent diffusion coefficient. After the injection of gadoxetic acid, (E) the lesion appeared heterogeneously hyperintense during the early phase and (F) relatively hypointense during the delayed phase. HIS, intrahepatic splenosis.

Radiographic features suggested a differential diagnosis of HCC, hepatic hemangioma, and hepatocellular adenoma. Surgical intervention was proposed, and the patient decided to undergo a laparoscopic surgery. During surgery, the tumor was found in segment 7 of the right lobe (Fig. 3A). The tumor's feeding artery and drainage vessel were found to be from the inferior phrenic artery and vein (Fig. 3B). Liver tumor was successfully resected laparoscopically. Then, histopathologic examination of the resected specimen revealed that the tumor was totally consisted of splenic tissue surrounded by a fibrous capsule (Fig. 4A and B). The postoperative diagnosis was confirmed as IHS. The patient recovered uneventfully and was discharged on the fourth postoperative day. The patient visited outpatient care for 2 years of postoperative follow-up without any trouble.

Figure 3 During the surgery. (A) Tumor was buried in the liver and adherent to the diaphragm. It had a capsule and was soft texture. (B) Drainage vessels of the tumor were recognized from the inferior phrenic artery and vein.

Figure 4 Histopathologic findings. (A) Resected specimen was dark red and 2.7 cm in size. (B) Hematoxylin and eosin staining (magnification, x20). The resected specimen was composed of white and red pulp surrounded by a fibrous capsule (W: white pulp, R: red pulp). Scale bar, 200 µM.

Discussion

Splenosis is usually caused by heterotopic autotransplantation or implantation of splenic tissue after elective splenectomy or traumatic splenic rupture. Approximately 70% of patients with IHS are reported to have a history of splenic rupture or surgery (11). IHS is rare because splenosis usually occurs in the mesentery, omentum, or peritoneum in the left upper abdomen (12).

After a literature search, using the search term ‘intrahepatic splenosis’ on the PubMed, we identified and reviewed 52 cases. Of these, 14 cases were excluded because of lacking clinical details, and the remaining 38 cases were included in our review (Table SI). There were 31 (81.5%) male and 7 (18.4%) female patients, with a mean age of 47.9 (±13) years. There was a previous history of abdominal trauma in 89% of the patients, and 97% had a history of splenectomy, which is consistent with a previous report (12). Seventy-three percent of patients were asymptomatic on admission. The mean tumor size on imaging was 3.7 (±1.2) cm. Fifty-eight percent of the patients underwent surgery, including hepatectomy, with the preoperative diagnosis of HCC or liver metastases (3,7,10,13).

The radiographic appearance of IHS generally varies. Typical findings are hypodense areas on non-contrast CT. Following contrast administration, the lesions are hyperdense in the arterial phase, iso-dense in the portal venous phase, and hypodense in the delayed phase (3,14). On MRI, IHS is usually described as areas of homogeneous hypo-intensity in T1WI and hyperintensity in T2WI (10). Following contrast administration, IHS shows a heterogeneous enhancement in the arterial phase, which becomes homogeneous in the later phases (8,9,15). In the delayed phase, the signal intensity of IHS may be lower than that of the liver parenchyma (10). The imaging features are similar to the signal and enhancement patterns of the spleen, usually described as geographic or zebra patterns of enhancement. In the previous studies, 61.5% of patients had typical radiological features. In our case, the enhancement pattern of the tumor in the enhanced MRI showed early enhancement in the arterial phase and washout in the delayed phase that was similar to the radiographic features of moderately differentiated HCC.

There are currently reported to be two dominant mechanisms of IHS occurring. One hypothesis is that an invagination or an exophytic growth of splenic tissue directly seeded into the liver capsule. With this mechanism, the most frequent site of IHS is the area surrounded by the left lobe and the diaphragm because it can be easily seeded with splenic tissue during splenectomy (14,16-18). The other is that hematogenous spread due to the entry of an erythrocyte progenitor cell into the portal venous system. In the latter pattern, the margin between IHS and liver parenchyma is usually unclear (2,19,20). In our case, tumor happened to metastasize in the right lobe near the inferior phrenic vessels without seeding at the most frequent site, and it was extrahepatically fed by these vessels. The tumor showed early enhancement in the arterial phase and washout in the delayed phase that was characteristic imaging pattern for HCC, although IHS shows a heterogeneous enhancement in the arterial phase, and homogeneous in the later phases (8,9,15). In our case, IHS was extrahepatically fed, so that the radiological findings were typical for HCC. It was difficult to make a correct diagnosis of IHS preoperatively. Surgical procedure was successfully performed.

We reported a rare case of IHS mimicking HCC. With a history of traumatic rupture of the spleen and splenectomy, even when the tumor has typical radiological patterns for HCC, IHS might be a different diagnosis.

Supplementary Material

Previous reports of patients with intrahepatic splenosis.

Acknowledgements

Not applicable.

Funding

Funding: No funding was received.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors' contributions

IU, HT, KM, AK, SM, RK, KN, SN, YY and AM participated in the diagnosis and treatment of the patient and wrote the first draft of this manuscript. All authors have read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Written informed consent was obtained from the patient to publish this case report and any accompanying images.

Competing interests

The authors declare that they have no competing interests.

References