Functional significance of B7-H1 expressed by human uveal melanoma cells

  • Authors:
    • Renbing Jia
    • Zhijun Jiao
    • Xiaofang Xu
    • Jing Wang
    • Yixiong Zhou
    • Xin Song
    • Shengfang Ge
    • Xianqun Fan
  • View Affiliations

  • Published online on: November 17, 2010     https://doi.org/10.3892/mmr.2010.397
  • Pages:163-165
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Abstract

B7-H1, a recently described B7 family member, has been reported to negatively regulate T-cell function in most cancer cells. In this study, we sought to investigate B7-H1 expression in four uveal melanoma (UM) cells (OCM1, SP6.5, OM431 and VUP) to determine the functional significance of B7-H1 expression in T-cell immune response. Using flow cytometry (FCM), we demonstrated that SP6.5 cells had high B7-H1 protein expression, while the other three UM cell lines had none. However, all four UM cell lines expressed B7-H1 mRNA, as confirmed by reverse transcription-polymerase chain reaction. In co-culture experiments using B7-H1-expressing UM cells with T-cells, FCM to determine CD69 expression in T-cells revealed that SP6.5 cell-related B7-H1 inhibited T-cell activation. This effect was eliminated by B7-H1-targeted RNA interference. An Annexin V/PI double staining assay further showed that B7-H1 expressed by SP6.5 cells did not increase the apoptosis of T-cells, though it was found in a variety of other solid tumors. In conclusion, all the UM cell lines constitutively expressed B7-H1 mRNA, while B7-H1 protein was expressed at different levels. UM-related B7-H1 expression negatively regulated T-cell immune response through the inhibition of T-cell activation, and not through the promotion of T-cell apoptosis. This provides new insight into anti-tumor immunity against B7-H1-expressing UM cells.

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January 2011
Volume 4 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Jia, R., Jiao, Z., Xu, X., Wang, J., Zhou, Y., Song, X. ... Fan, X. (2011). Functional significance of B7-H1 expressed by human uveal melanoma cells. Molecular Medicine Reports, 4, 163-165. https://doi.org/10.3892/mmr.2010.397
MLA
Jia, R., Jiao, Z., Xu, X., Wang, J., Zhou, Y., Song, X., Ge, S., Fan, X."Functional significance of B7-H1 expressed by human uveal melanoma cells". Molecular Medicine Reports 4.1 (2011): 163-165.
Chicago
Jia, R., Jiao, Z., Xu, X., Wang, J., Zhou, Y., Song, X., Ge, S., Fan, X."Functional significance of B7-H1 expressed by human uveal melanoma cells". Molecular Medicine Reports 4, no. 1 (2011): 163-165. https://doi.org/10.3892/mmr.2010.397