Multiplexed profiling of secreted proteins for the detection of potential space biomarkers

  • Authors:
    • Birger Dieriks
    • Winnok H. De Vos
    • Marjan Moreels
    • Myriam Ghardi
    • Raoul Hennekam
    • Jos L.V. Broers
    • Sarah Baatout
    • Patrick van Oostveldt
  • View Affiliations

  • Published online on: November 30, 2010     https://doi.org/10.3892/mmr.2010.405
  • Pages:17-23
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Space travel exposes astronauts to a plethora of potentially detrimental conditions, such as cosmic radiation and microgravity. As both factors are hard to simulate on Earth, present knowledge remains limited. However, this knowledge is of vital importance, making space flight experiments a necessity for determining the biological effects and the underlying biochemical processes, especially when keeping future long-term interplanetary missions in mind. Instead of estimating the long-term effects, which usually implicate severe endpoints (e.g., cancer) and which are often difficult to attribute, research has shifted to finding representative biomarkers for rapid and sensitive detection of individual radiosensitivity. In this context, an appealing set of candidate markers is the group of secreted proteins, as they exert an intercellular signaling function and are easy to assess. We screened a subset of secreted proteins in cells exposed to space travel by means of multiplex bead array analysis. To determine the cell-specific signatures of the secreted molecules, we compared the conditioned medium of normal fibroblast cells to fibroblasts isolated from a patient with Hutchinson-Gilford Progeria syndrome, which are known to have a perturbed nuclear architecture and DNA damage response. Out of the 88 molecules screened, 20 showed a significant level increase or decrease, with a differential response to space conditions between the two cell types. Among the molecules that were retained, which may prove to be valuable biomarkers, are apolipoprotein C-III, plasminogen activator inhibitor type 1, β-2-microglobulin, ferritin, MMP-3, TIMP-1 and VEGF.

Related Articles

Journal Cover

January 2011
Volume 4 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Dieriks, B., De Vos, W.H., Moreels, M., Ghardi, M., Hennekam, R., Broers, J.L. ... van Oostveldt, P. (2011). Multiplexed profiling of secreted proteins for the detection of potential space biomarkers. Molecular Medicine Reports, 4, 17-23. https://doi.org/10.3892/mmr.2010.405
MLA
Dieriks, B., De Vos, W. H., Moreels, M., Ghardi, M., Hennekam, R., Broers, J. L., Baatout, S., van Oostveldt, P."Multiplexed profiling of secreted proteins for the detection of potential space biomarkers". Molecular Medicine Reports 4.1 (2011): 17-23.
Chicago
Dieriks, B., De Vos, W. H., Moreels, M., Ghardi, M., Hennekam, R., Broers, J. L., Baatout, S., van Oostveldt, P."Multiplexed profiling of secreted proteins for the detection of potential space biomarkers". Molecular Medicine Reports 4, no. 1 (2011): 17-23. https://doi.org/10.3892/mmr.2010.405