Protective role of autophagy in AGE-induced early injury of human vascular endothelial cells

  • Authors:
    • Ying Xie
    • Shou-Jiang You
    • Yan-Lin Zhang
    • Qiao Han
    • Yong-Jun  Cao
    • Xing-Shun Xu
    • Ya-Ping Yang
    • Jun Li
    • Chun-Feng Liu
  • View Affiliations

  • Published online on: March 22, 2011     https://doi.org/10.3892/mmr.2011.460
  • Pages: 459-464
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Abstract

Advanced glycation end-products (AGEs) contribute to the pathogenesis of diabetes mellitus and atherosclerosis by promoting vascular endothelial cell proliferation, migration, damage and death. In this study, we examined the role of autophagy in HUVECs exposed to AGE-modified bovine serum albumin (AGE-BSA). HUVECs incubated with AGE-BSA for 6 h showed an increase in the formation of acidic vesicular organelles and autophagosomes. AGE-BSA-induced upregulation of microtubule associated protein 1 light chain 3-II (LC3-II), a marker of autophagy, was abolished by pretreatment with the autophagy inhibitor 3-methyladenine (3-MA), and was increased by rapamycin, an autophagy inducer. The increase of lactate dehydrogenase (LDH) leakage induced by AGE-BSA was increased by 3-MA, but not rapamycin. An oxidative inhibitor, α-tocopherol, decreased not only the AGE-BSA-induced increase of reactive oxygen species, but also the upregulation of LC3-II protein levels. These results suggest that AGE-BSA increases the level of autophagy, which is protective against HUVEC injury, and that ROS play a role in this activation of autophagy.

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May 2011
Volume 4 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Xie, Y., You, S., Zhang, Y., Han, Q., Cao, Y., Xu, X. ... Liu, C. (2011). Protective role of autophagy in AGE-induced early injury of human vascular endothelial cells. Molecular Medicine Reports, 4, 459-464. https://doi.org/10.3892/mmr.2011.460
MLA
Xie, Y., You, S., Zhang, Y., Han, Q., Cao, Y., Xu, X., Yang, Y., Li, J., Liu, C."Protective role of autophagy in AGE-induced early injury of human vascular endothelial cells". Molecular Medicine Reports 4.3 (2011): 459-464.
Chicago
Xie, Y., You, S., Zhang, Y., Han, Q., Cao, Y., Xu, X., Yang, Y., Li, J., Liu, C."Protective role of autophagy in AGE-induced early injury of human vascular endothelial cells". Molecular Medicine Reports 4, no. 3 (2011): 459-464. https://doi.org/10.3892/mmr.2011.460