Open Access

A novel chimeric DNA vaccine: Enhancement of preventive and therapeutic efficacy of DNA vaccine by fusion of Mucin 1 to a heat shock protein 70 gene

  • Authors:
    • Dae-Han Choi
    • Jong Kyu Woo
    • Yun Choi
    • Hye-Sook Seo
    • Chul-Woo Kim
  • View Affiliations

  • Published online on: Friday, July 1, 2011
  • Pages:885-890 DOI: 10.3892/mmr.2011.525
0

Abstract

Intensive efforts to improve vaccines against cancer are currently outgoing. Mucin 1 (Muc1) is a tumor-specific antigen that is overexpressed and heavily glycosylated in a variety of adenocarcinomas. In the present study, the efficacy of an anticancer DNA vaccination strategy was demonstrated using Muc1 fusion vaccines. To enhance antigen presentation and tumor-suppressive efficacy, a chimeric Muc1 vaccine was designed, encoding the transmembrane- and C-terminal domain-deleted Muc1 gene (∆TM) fused to the human HSP70 gene. To confirm the expression and secretion of fusion protein, cell culture supernatants were subjected to Western blotting. We found secreted Muc1 ΔTM-HSP0 fusion protein in the supernatants. These results demonstrate that the Muc1 ΔTM-HSP0 construct can be efficiently expressed and secreted from transfected cells. When the chimeric Muc1 vaccine was administered to mice, antigen-specific cellular immune responses were observed. Notably, we observed that antigen-specific lymphocyte proliferation and cytotoxic responses were effectively induced only in the group of mice that had been vaccinated with the chimeric Muc1 vaccine. Concurrent with the Muc1-specific tumor-suppressive effect, the growth of established Muc1-expressing B16 mouse melanoma cells was also significantly inhibited by vaccination with the chimeric Muc1 vaccine. The growth of B16 mouse melanoma cells expressing human Muc1 in C57BL/6 mice was effectively suppressed by the Muc1-HSP70 chimeric DNA vaccine. Our results reveal that the antitumor efficacy of the chimeric DNA vaccine was improved by the presence of HSP/70.

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Journal Cover

September 2011
Volume 4 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

2015 Impact Factor: 1.559
Ranked #48/123 Medicine Research and Experimental
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APA
Choi, D., Woo, J., Choi, Y., Seo, H., & Kim, C. (2011). A novel chimeric DNA vaccine: Enhancement of preventive and therapeutic efficacy of DNA vaccine by fusion of Mucin 1 to a heat shock protein 70 gene. Molecular Medicine Reports, 4, 885-890. http://dx.doi.org/10.3892/mmr.2011.525
MLA
Choi, D., Woo, J., Choi, Y., Seo, H., Kim, C."A novel chimeric DNA vaccine: Enhancement of preventive and therapeutic efficacy of DNA vaccine by fusion of Mucin 1 to a heat shock protein 70 gene". Molecular Medicine Reports 4.5 (2011): 885-890.
Chicago
Choi, D., Woo, J., Choi, Y., Seo, H., Kim, C."A novel chimeric DNA vaccine: Enhancement of preventive and therapeutic efficacy of DNA vaccine by fusion of Mucin 1 to a heat shock protein 70 gene". Molecular Medicine Reports 4, no. 5 (2011): 885-890. http://dx.doi.org/10.3892/mmr.2011.525