Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cells

  • Authors:
    • Wei Zhang
    • Yanqiu Fang
    • Xu Shi
    • Minglei Zhang
    • Xiaoqi Wang
    • Yan Tan
  • View Affiliations

  • Published online on: September 9, 2011     https://doi.org/10.3892/mmr.2011.583
  • Pages: 41-47
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Abstract

The aim of this study was to explore the effect of bisphenol A (BPA) on the EGFR-STAT3 pathway in breast cancer. We applied 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity assay to the analysis of the responsiveness of MCF-7 cells to BPA. Gene expression was assayed at the transcriptional and translational levels by reverse transcription-PCR and Western blotting. We explored the effects of BPA on MCF-7 cell prolife­ration through inhibition of the related genes, STAT3, using RNA interference, and EGFR, using its inhibitor AG1478. The optimal concentration and time point of BPA-induced proli­feration in MCF-7 cells are 1 µM and 24 h, respectively. BPA significantly increased the expression of STAT3 at a concentration of 1 µM following treatment for 48 h and the expression of STAT3 was down-regulated after blocking EGFR. When STAT3 was blocked in MCF-7 cells, BPA did not appear to induce cell proliferation. Treatment with BPA (1 µM) in the presence of AG1478 for 48 h resulted in the stimulation of cell growth in MCF-7 cells, similar to that of the BPA alone treatment. BPA increases STAT3 expression, which is a major factor in the pathway of BPA-induced proliferation, and STAT3 activation contributes to BPA-induced breast cancer cell proliferation. However, EGFR mediates negative signaling for BPA-induced breast cancer cell proliferation.

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January 2012
Volume 5 Issue 1

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Spandidos Publications style
Zhang W, Fang Y, Shi X, Zhang M, Wang X and Tan Y: Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cells. Mol Med Rep 5: 41-47, 2012
APA
Zhang, W., Fang, Y., Shi, X., Zhang, M., Wang, X., & Tan, Y. (2012). Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cells. Molecular Medicine Reports, 5, 41-47. https://doi.org/10.3892/mmr.2011.583
MLA
Zhang, W., Fang, Y., Shi, X., Zhang, M., Wang, X., Tan, Y."Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cells". Molecular Medicine Reports 5.1 (2012): 41-47.
Chicago
Zhang, W., Fang, Y., Shi, X., Zhang, M., Wang, X., Tan, Y."Effect of bisphenol A on the EGFR-STAT3 pathway in MCF-7 breast cancer cells". Molecular Medicine Reports 5, no. 1 (2012): 41-47. https://doi.org/10.3892/mmr.2011.583