Expression levels of microRNA-375 in colorectal carcinoma

  • Authors:
    • Xiaoning Dai
    • Yeunpo Chiang
    • Zhenning Wang
    • Yongxi Song
    • Chong Lu
    • Peng Gao
    • Huimian Xu
  • View Affiliations

  • Published online on: February 29, 2012     https://doi.org/10.3892/mmr.2012.815
  • Pages: 1299-1304
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Abstract

MicroRNAs are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have altered expressions in many cancer types. The expression levels of miR-375 have not been comprehensively investigated in colorectal cancer. In this study, total RNA was extracted from 95 pairs of colorectal cancer tissues and non-tumor adjacent tissues, as well as from three colorectal cancer cell lines (HT-29, HCT-116 and SW-620). After polyadenylation and reverse transcription, we determined the expression levels of miR-375 by real-time PCR and calculated the difference in expression using the 2-∆∆Ct method. We assessed the correlation between the expression levels of miR-375 and clini­copathological characteristics of colorectal cancer. miR-375 expression was frequently downregulated in the colorectal cancer tissues compared to the non-tumor counterparts (p<0.001; paired t-test). Moreover, a significantly low expression of miR-375 was also found in the colorectal cancer cell lines (HT-29, p=0.002; HCT-116, p<0.001; SW-620, p=0.004; paired t-test). However, there were no significant correlations between the low expression of miR-375 and tumor size, histological grade, pT stage, pN stage and pTNM stage (all P>0.05, non-parametric test; Mann-Whitney U test between two groups and Kruskal-Wallis H test for three or more groups). miR-375 may be involved in the carcinogenesis of colorectal cancers and may be a potential biomarker for colorectal cancers.

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May 2012
Volume 5 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Dai, X., Chiang, Y., Wang, Z., Song, Y., Lu, C., Gao, P., & Xu, H. (2012). Expression levels of microRNA-375 in colorectal carcinoma. Molecular Medicine Reports, 5, 1299-1304. https://doi.org/10.3892/mmr.2012.815
MLA
Dai, X., Chiang, Y., Wang, Z., Song, Y., Lu, C., Gao, P., Xu, H."Expression levels of microRNA-375 in colorectal carcinoma". Molecular Medicine Reports 5.5 (2012): 1299-1304.
Chicago
Dai, X., Chiang, Y., Wang, Z., Song, Y., Lu, C., Gao, P., Xu, H."Expression levels of microRNA-375 in colorectal carcinoma". Molecular Medicine Reports 5, no. 5 (2012): 1299-1304. https://doi.org/10.3892/mmr.2012.815