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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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June 2012 Volume 5 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells

  • Authors:
    • Christina M. Hardaway
    • Ramesh B. Badisa
    • Karam F.A. Soliman
  • View Affiliations / Copyright

    Affiliations: Meharry Medical College, Nashville, TN 37208, USA, College of Pharmacy and Pharmaceutical Sciences, Florida A__AMB__M University, Tallahassee, FL 32307, USA
  • Pages: 1449-1452
    |
    Published online on: April 2, 2012
       https://doi.org/10.3892/mmr.2012.857
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Abstract

Ascorbic acid is one of the antioxidant compounds widely used against free radical stress. The present study was undertaken to examine whether ascorbic acid and hydrogen peroxide (H2O2), alone or in combination, could influence cell viability. The murine neuroblastoma cell line, N2a, was used to perform a dose response curve for ascorbic acid. It was observed that ascorbic acid alone at physiological concentrations (0.1-0.4 mM) did not cause any cell death. However, at pharmacological concentrations (1-6 mM), ascorbic acid caused dose-dependent cell death. The lethal concentration at which 50% cells were killed (LC50) was determined to be approximately 3.141 mM ascorbic acid at 24 h. H2O2 up to 300 µM alone did not cause significant cell death. In the combined treatment, when the cells were treated with ascorbic acid at physiological concentrations (0.4 mM) and H2O2 at 400 µM, higher rates of cell death were observed compared to the cell death rates caused by either compound alone. Subsequent experiments revealed that cell death was partly mediated through the loss of total glutathione levels in the cells. These data suggest that the combination of ascorbic acid and H2O2 is disadvantageous for cancer cell survival. Further studies are required to ascertain the physiological significance of these observations.

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Copy and paste a formatted citation
Spandidos Publications style
Hardaway CM, Badisa RB and Soliman KF: Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells. Mol Med Rep 5: 1449-1452, 2012.
APA
Hardaway, C.M., Badisa, R.B., & Soliman, K.F. (2012). Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells. Molecular Medicine Reports, 5, 1449-1452. https://doi.org/10.3892/mmr.2012.857
MLA
Hardaway, C. M., Badisa, R. B., Soliman, K. F."Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells". Molecular Medicine Reports 5.6 (2012): 1449-1452.
Chicago
Hardaway, C. M., Badisa, R. B., Soliman, K. F."Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells". Molecular Medicine Reports 5, no. 6 (2012): 1449-1452. https://doi.org/10.3892/mmr.2012.857
Copy and paste a formatted citation
x
Spandidos Publications style
Hardaway CM, Badisa RB and Soliman KF: Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells. Mol Med Rep 5: 1449-1452, 2012.
APA
Hardaway, C.M., Badisa, R.B., & Soliman, K.F. (2012). Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells. Molecular Medicine Reports, 5, 1449-1452. https://doi.org/10.3892/mmr.2012.857
MLA
Hardaway, C. M., Badisa, R. B., Soliman, K. F."Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells". Molecular Medicine Reports 5.6 (2012): 1449-1452.
Chicago
Hardaway, C. M., Badisa, R. B., Soliman, K. F."Effect of ascorbic acid and hydrogen peroxide on mouse neuroblastoma cells". Molecular Medicine Reports 5, no. 6 (2012): 1449-1452. https://doi.org/10.3892/mmr.2012.857
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