MicroRNA‑494 inhibits nerve growth factor‑induced cell proliferation by targeting cyclin D1 in human corneal epithelial cells

  • Authors:
    • Dan Wu
    • Tingting Qian
    • Jiaxu Hong
    • Gang Li
    • Weiyun Shi
    • Jianjiang Xu
  • View Affiliations

  • Published online on: July 25, 2017     https://doi.org/10.3892/mmr.2017.7083
  • Pages: 4133-4142
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Nerve growth factor (NGF) is expressed in the human corneal epithelium and stroma. It is an efficient therapy for human corneal ulcers caused by neurotropic disease. However, little is known about the molecular mechanism of NGF in healing human corneal epithelial diseases. Numerous microRNAs (miRNAs) are expressed in the cornea and miRNAs have important roles in regulating corneal development. In the present study, novel miRNA regulators were demonstrated to be involved in NGF‑induced human corneal epithelial cell (hCEC) proliferation. NGF treatment significantly downregulated the expression of miRNA‑494 in hCECs in vitro. Furthermore, miRNA‑494 increased G1 arrest in the immortalized human corneal epithelial cell (ihCEC) line and suppressed cell proliferation. Accordingly, bioinformatics programs and luciferase reporter assay demonstrated that miRNA‑494 directly targeted cyclin D1 by binding to a sequence in the 3'‑untranslated region. In addition, overexpression of miRNA‑494 decreased both basal and NGF‑induced cyclin D1 expression. NGF treatment partially suppressed miRNA‑494 expression and restored cyclin D1 expression. Furthermore, co‑transfection of miRNA‑494 with the cyclin D1 ORF clone partially restored cyclin D1 mRNA and protein expression. These findings indicate that miRNA‑494 and its target cyclin D1 may be a crucial axis for NGF in regulating the proliferation of hCEC. Specific modulation of miRNA‑494 in hCEC could represent an attractive approach for treating cornea epithelial diseases.

Related Articles

Journal Cover

October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wu D, Qian T, Hong J, Li G, Shi W and Xu J: MicroRNA‑494 inhibits nerve growth factor‑induced cell proliferation by targeting cyclin D1 in human corneal epithelial cells. Mol Med Rep 16: 4133-4142, 2017
APA
Wu, D., Qian, T., Hong, J., Li, G., Shi, W., & Xu, J. (2017). MicroRNA‑494 inhibits nerve growth factor‑induced cell proliferation by targeting cyclin D1 in human corneal epithelial cells. Molecular Medicine Reports, 16, 4133-4142. https://doi.org/10.3892/mmr.2017.7083
MLA
Wu, D., Qian, T., Hong, J., Li, G., Shi, W., Xu, J."MicroRNA‑494 inhibits nerve growth factor‑induced cell proliferation by targeting cyclin D1 in human corneal epithelial cells". Molecular Medicine Reports 16.4 (2017): 4133-4142.
Chicago
Wu, D., Qian, T., Hong, J., Li, G., Shi, W., Xu, J."MicroRNA‑494 inhibits nerve growth factor‑induced cell proliferation by targeting cyclin D1 in human corneal epithelial cells". Molecular Medicine Reports 16, no. 4 (2017): 4133-4142. https://doi.org/10.3892/mmr.2017.7083