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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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October-2017 Volume 16 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects

  • Authors:
    • Aijing Zhu
    • Xiuyun Wang
    • Min Huang
    • Chen Chen
    • Juan Yan
    • Qi Xu
    • Lijia Wei
    • Xianzhou Huang
    • Hong Zhu
    • Cheng Yi

  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Laboratory of Chengdu Huachuang Biotechnology Co., Ltd., Chengdu, Sichuan 610000, P.R. China
  • Pages: 4973-4979
    |
    Published online on: August 2, 2017
       https://doi.org/10.3892/mmr.2017.7146
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Abstract

TNF ligand superfamily member 10 (TRAIL) is a member of the tumor necrosis factor superfamily. The present study was performed in an effort to increase the expression of soluble (s)TRAIL by rebuilding the gene sequence of TRAIL. Three principles based on the codon bias of Escherichia coli were put forward to design the rebuild strategy. Relying on these three principles, a P7R mutation near the N‑terminal region of sTRAIL, named TRAIL‑Mu, was designed. TRAIL‑Mu was subsequently cloned into the PTWIN1 plasmid and expressed in E. coli BL21 (DE3). Using a high‑level expression system and a three‑step purification method, soluble TRAIL‑Mu protein reached ~90% of total cellular protein and purity was >95%, demonstrating success in overcoming inclusion body formation. The cytotoxic effect of TRAIL‑Mu was evaluated by sulforhodamine B assay in the MD‑MB‑231, A549, NCI‑H460 and L02 cell lines. The results demonstrated that TRAIL‑Mu exerted stronger antitumor effects on TRAIL‑sensitive tumor cell lines, and was able to partially reverse the resistance of a TRAIL‑resistant tumor cell line. In addition, TRAIL‑Mu exhibited no notable biological effects in a normal liver cell line. The novel TRAIL variant generated in the present study may be useful for the mass production of this important protein for therapeutic purposes.

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Copy and paste a formatted citation
Spandidos Publications style
Zhu A, Wang X, Huang M, Chen C, Yan J, Xu Q, Wei L, Huang X, Zhu H, Yi C, Yi C, et al: Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects. Mol Med Rep 16: 4973-4979, 2017.
APA
Zhu, A., Wang, X., Huang, M., Chen, C., Yan, J., Xu, Q. ... Yi, C. (2017). Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects. Molecular Medicine Reports, 16, 4973-4979. https://doi.org/10.3892/mmr.2017.7146
MLA
Zhu, A., Wang, X., Huang, M., Chen, C., Yan, J., Xu, Q., Wei, L., Huang, X., Zhu, H., Yi, C."Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects". Molecular Medicine Reports 16.4 (2017): 4973-4979.
Chicago
Zhu, A., Wang, X., Huang, M., Chen, C., Yan, J., Xu, Q., Wei, L., Huang, X., Zhu, H., Yi, C."Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects". Molecular Medicine Reports 16, no. 4 (2017): 4973-4979. https://doi.org/10.3892/mmr.2017.7146
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu A, Wang X, Huang M, Chen C, Yan J, Xu Q, Wei L, Huang X, Zhu H, Yi C, Yi C, et al: Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects. Mol Med Rep 16: 4973-4979, 2017.
APA
Zhu, A., Wang, X., Huang, M., Chen, C., Yan, J., Xu, Q. ... Yi, C. (2017). Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects. Molecular Medicine Reports, 16, 4973-4979. https://doi.org/10.3892/mmr.2017.7146
MLA
Zhu, A., Wang, X., Huang, M., Chen, C., Yan, J., Xu, Q., Wei, L., Huang, X., Zhu, H., Yi, C."Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects". Molecular Medicine Reports 16.4 (2017): 4973-4979.
Chicago
Zhu, A., Wang, X., Huang, M., Chen, C., Yan, J., Xu, Q., Wei, L., Huang, X., Zhu, H., Yi, C."Generation of a novel TRAIL mutant by proline to arginine substitution based on codon bias and its antitumor effects". Molecular Medicine Reports 16, no. 4 (2017): 4973-4979. https://doi.org/10.3892/mmr.2017.7146
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