Open Access

MicroRNA‑643 regulates the expression of ZEB1 and inhibits tumorigenesis in osteosarcoma

Retraction in: /10.3892/mmr.2022.12792

  • Authors:
    • Huan Wang
    • Danmou Xing
    • Dong Ren
    • Wei Feng
    • Yan Chen
    • Zhiming Zhao
    • Zhihong Xiao
    • Zhengren Peng
  • View Affiliations

  • Published online on: August 17, 2017     https://doi.org/10.3892/mmr.2017.7273
  • Pages: 5157-5164
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is among the most malignant types of tumor worldwide and has become a leading contributor to tumor incidence, particularly in adolescents. Resistance to conventional treatment and the complexity of osteosarcoma tumorigenesis has resulted in high mortality rates. MicroRNAs are a class of noncoding RNAs, which regulate numerous biological processes. However, the involvement of miR‑643 in osteosarcoma remains to be elucidated. In the present study, reverse transcription‑quantitative polymerase chain reaction, luciferase reporter assay, invasion assay, viability assay, western blot analysis and in vivo implantation were performed to analyze the action of miR‑643 in osteosarcoma. The results demonstrated that miR‑643 inhibited the progression of osteosarcoma and acted as a potential tumor suppressor. The expression of miR‑643 was downregulated in osteosarcoma tissues and cell lines. In addition, miR‑643 transfection significantly impaired the proliferation and invasion of osteosarcoma cells. The present study also identified Zinc finger E‑box‑binding homeobox 1 (ZEB1) as a direct target of miR‑643, and the ectopic expression of ZEB1 counteracted the effect of miR‑643 transfection. A significant inverse correlation was also found between the expression of miR‑643 and ZEB1. A low expression of miR‑643 or a high expression of ZEB1 was associated with poor patient survival rates. The results of the present study suggested that the decreased expression of miR‑643 may be involved in the mechanism underlying the development of osteosarcoma. The intricate interactions between miR‑643 and ZEB1 may serve as a potential therapeutic target in osteosarcoma oncogenesis.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang H, Xing D, Ren D, Feng W, Chen Y, Zhao Z, Xiao Z and Peng Z: MicroRNA‑643 regulates the expression of ZEB1 and inhibits tumorigenesis in osteosarcoma Retraction in /10.3892/mmr.2022.12792. Mol Med Rep 16: 5157-5164, 2017.
APA
Wang, H., Xing, D., Ren, D., Feng, W., Chen, Y., Zhao, Z. ... Peng, Z. (2017). MicroRNA‑643 regulates the expression of ZEB1 and inhibits tumorigenesis in osteosarcoma Retraction in /10.3892/mmr.2022.12792. Molecular Medicine Reports, 16, 5157-5164. https://doi.org/10.3892/mmr.2017.7273
MLA
Wang, H., Xing, D., Ren, D., Feng, W., Chen, Y., Zhao, Z., Xiao, Z., Peng, Z."MicroRNA‑643 regulates the expression of ZEB1 and inhibits tumorigenesis in osteosarcoma Retraction in /10.3892/mmr.2022.12792". Molecular Medicine Reports 16.4 (2017): 5157-5164.
Chicago
Wang, H., Xing, D., Ren, D., Feng, W., Chen, Y., Zhao, Z., Xiao, Z., Peng, Z."MicroRNA‑643 regulates the expression of ZEB1 and inhibits tumorigenesis in osteosarcoma Retraction in /10.3892/mmr.2022.12792". Molecular Medicine Reports 16, no. 4 (2017): 5157-5164. https://doi.org/10.3892/mmr.2017.7273