The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients

  • Authors:
    • Kei Nanba
    • Shinichi Toyooka
    • Junichi Soh
    • Kazunori Tsukuda
    • Hiromasa Yamamoto
    • Akiko Sakai
    • Mamoru Ouchida
    • Naruyuki Kobayashi
    • Keitaro Matsuo
    • Norio Koide
    • Katsuyasu Kusaka
    • Kenji Shimizu
    • Hiroshi Date
  • View Affiliations

  • Published online on: September 1, 2008     https://doi.org/10.3892/mmr_00000010
  • Pages: 667-671
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Abstract

It has been reported that the rs1862214 single nucleotide polymorphism (SNP) in the programmed cell death 5 gene (PDCD5) is associated with smoking-related lung cancer risk and prognosis in a European population with a history of smoking. The aim of this study was to investigate the status and impact of SNPs in the PDCD5 locus of a Japanese population. We developed an assay based on real-time PCR with melting curve analysis for determining the rs1862214 SNP, and examined this SNP in 165 lung cancer patients and 180 healthy volunteers. Of the 165 lung cancer patients (107 smokers), 25 (17), 72 (47) and 68 (43) had the CC, CG and GG genotypes of rs1862214, respectively. Of the 180 volunteers (117 smokers), 31 (24), 81 (52) and 68 (41) had the CC, CG and GG genotypes of rs1862214, respectively. No significant difference in allelic distribution was found between Japanese patients and healthy controls, even among smokers. Based on the published data, the distribution of this SNP appears to be significantly different in Japanese and European populations. No significant difference in prognosis according to the SNP was observed, either in patients with a history of smoking or in the total number of patients. This too differs from the results from a European population. In conclusion, we developed a convenient real-time PCR-based assay for the genotyping of rs1862214 in the PDCD5 locus. The distribution of the rs1862214 SNP in our Japanese population differs from its distribution in a European population, and is not related to the risk of cancer or to poor prognosis in lung cancer. This suggests the presence of an ethnicity-related difference in the role of PDCD5 in the pathogenesis of lung cancer.

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September-October 2008
Volume 1 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Nanba K, Toyooka S, Soh J, Tsukuda K, Yamamoto H, Sakai A, Ouchida M, Kobayashi N, Matsuo K, Koide N, Koide N, et al: The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients. Mol Med Rep 1: 667-671, 2008
APA
Nanba, K., Toyooka, S., Soh, J., Tsukuda, K., Yamamoto, H., Sakai, A. ... Date, H. (2008). The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients. Molecular Medicine Reports, 1, 667-671. https://doi.org/10.3892/mmr_00000010
MLA
Nanba, K., Toyooka, S., Soh, J., Tsukuda, K., Yamamoto, H., Sakai, A., Ouchida, M., Kobayashi, N., Matsuo, K., Koide, N., Kusaka, K., Shimizu, K., Date, H."The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients". Molecular Medicine Reports 1.5 (2008): 667-671.
Chicago
Nanba, K., Toyooka, S., Soh, J., Tsukuda, K., Yamamoto, H., Sakai, A., Ouchida, M., Kobayashi, N., Matsuo, K., Koide, N., Kusaka, K., Shimizu, K., Date, H."The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients". Molecular Medicine Reports 1, no. 5 (2008): 667-671. https://doi.org/10.3892/mmr_00000010