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Up-regulation of the G3PDH ‘housekeeping’ gene by estrogen

  • Authors:
    • Nadia Galal
    • Waleed El-Beialy
    • Yoshiaki Deyama
    • Yoshitaka Yoshimura
    • Kanchu Tei
    • Kuniaki Suzuki
    • Yasunori Totsuka

  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000226
  • Pages: 111-113
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Abstract

Proteomic and genomic studies commonly involve the assessment of mRNA levels using reverse transcription-polymerase chain reaction (PCR) and real-time quantitative PCR. An internal standard RNA is fundamentally analyzed along with the investigated mRNA to document the specificity of the effect(s) on mRNA and to correct for inter-sample variations. In our studies implementing estrogen treatments on different cell lines, we initially used glyceraldehyde-3-phosphate dehydrogenase (G3PDH) as an internal standard. However, the results of PCR amplification demonstrated that 17β-estradiol enhanced the expression of the G3PDH gene, rendering it impossible to use G3PDH as an unbiased comparative control.

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January-February 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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  • Journal Metrics
  • Impact Factor: 3.4
  • Ranked Medicine Research and Experimental
    (total number of cites)
  • CiteScore: 6.9
  • CiteScore Rank: Q2: #98/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.721
  • SCImago Journal Rank (SJR): 0.65
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Spandidos Publications style
Galal N, El-Beialy W, Deyama Y, Yoshimura Y, Tei K, Suzuki K and Totsuka Y: Up-regulation of the G3PDH ‘housekeeping’ gene by estrogen . Mol Med Rep 3: 111-113, 2010
APA
Galal, N., El-Beialy, W., Deyama, Y., Yoshimura, Y., Tei, K., Suzuki, K., & Totsuka, Y. (2010). Up-regulation of the G3PDH ‘housekeeping’ gene by estrogen . Molecular Medicine Reports, 3, 111-113. https://doi.org/10.3892/mmr_00000226
MLA
Galal, N., El-Beialy, W., Deyama, Y., Yoshimura, Y., Tei, K., Suzuki, K., Totsuka, Y."Up-regulation of the G3PDH ‘housekeeping’ gene by estrogen ". Molecular Medicine Reports 3.1 (2010): 111-113.
Chicago
Galal, N., El-Beialy, W., Deyama, Y., Yoshimura, Y., Tei, K., Suzuki, K., Totsuka, Y."Up-regulation of the G3PDH ‘housekeeping’ gene by estrogen ". Molecular Medicine Reports 3, no. 1 (2010): 111-113. https://doi.org/10.3892/mmr_00000226