Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor

  • Authors:
    • Soetkin Versteyhe
    • Christophe Blanquart
    • Cornelia Hampe
    • Shaukat Mahmood
    • Nevena Christeff
    • Pierre De Meyts
    • Steven G. Gray
    • Tarik Issad
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/mmr_00000239
  • Pages: 189-193
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Abstract

Insulin receptor substrates (IRS)-5 and -6 are two recently identified members of the IRS family. We investigated their roles as insulin receptor substrates and compared them with Src-homology-2-containing (Shc) protein, a well-established substrate. Bioluminescence resonance energy transfer (BRET) experiments showed no interaction between the receptor and IRS-5, while interaction with IRS-6 was not enhanced by insulin. By contrast, Shc showed an insulin-induced BRET response, as did a truncated form of IRS-1 (1-262). While Shc was heavily phosphorylated after stimulation of the insulin receptor, IRS-5 and -6 showed very weak phosphorylation levels. These results suggest that, although these two adaptors have previously been proposed as substrates for the insulin receptor, they are poor substrates for the insulin receptor. This calls into question their relevance to insulin signalling.

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January-February 2010
Volume 3 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Versteyhe S, Blanquart C, Hampe C, Mahmood S, Christeff N, De Meyts P, Gray SG and Issad T: Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor . Mol Med Rep 3: 189-193, 2010
APA
Versteyhe, S., Blanquart, C., Hampe, C., Mahmood, S., Christeff, N., De Meyts, P. ... Issad, T. (2010). Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor . Molecular Medicine Reports, 3, 189-193. https://doi.org/10.3892/mmr_00000239
MLA
Versteyhe, S., Blanquart, C., Hampe, C., Mahmood, S., Christeff, N., De Meyts, P., Gray, S. G., Issad, T."Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor ". Molecular Medicine Reports 3.1 (2010): 189-193.
Chicago
Versteyhe, S., Blanquart, C., Hampe, C., Mahmood, S., Christeff, N., De Meyts, P., Gray, S. G., Issad, T."Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor ". Molecular Medicine Reports 3, no. 1 (2010): 189-193. https://doi.org/10.3892/mmr_00000239