The MEK inhibitor PD98059 attenuates growth inhibition and death in gallic acid-treated Calu-6 lung cancer cells by preventing glutathione depletion

  • Authors:
    • Yong Hwan Han
    • Hwa Jin Moon
    • Bo Ra You
    • Yeon Mi Yang
    • Sung Zoo Kim
    • Suhn Hee Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/mmr_00000291
  • Pages: 519-525
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Abstract

Gallic acid (GA) is widely distributed in various plants and foods and has various biological effects. In this study, we investigated the effects of mitogen-activated protein kinase (MEK, JNK or p38) inhibitors on GA-induced Calu-6 lung cancer cell death in relation to reactive oxygen species (ROS) and glutathione (GSH) levels. GA inhibited the growth of Calu-6 cells and induced apoptosis and/or necrosis accompanied by the loss of mitochondrial membrane potential (MMP; Δψm). ROS levels and the number of GSH-depleted cells were observed to be increased at 24 h. MEK inhibitor suppressed cell growth inhibition, death, MMP (Δψm) loss and GSH depletion induced by GA, but failed to suppress the increase in ROS levels. JNK inhibitor also somewhat suppressed cell growth inhibition, MMP (Δψm) loss and GSH depletion induced by GA, and limited the increase in ROS levels. By contrast, p38 inhibitor mildly enhanced GA-induced cell growth inhibition, MMP (Δψm) loss and the increase in ROS levels. In conclusion, MEK inhibitor suppressed GA-induced cell growth inhibition and death in Calu-6 cells. This was related to the prevention of GSH depletion.

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May-June 2010
Volume 3 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Han YH, Moon HJ, You BR, Yang YM, Kim SZ, Kim SH and Park WH: The MEK inhibitor PD98059 attenuates growth inhibition and death in gallic acid-treated Calu-6 lung cancer cells by preventing glutathione depletion. Mol Med Rep 3: 519-525, 2010.
APA
Han, Y.H., Moon, H.J., You, B.R., Yang, Y.M., Kim, S.Z., Kim, S.H., & Park, W.H. (2010). The MEK inhibitor PD98059 attenuates growth inhibition and death in gallic acid-treated Calu-6 lung cancer cells by preventing glutathione depletion. Molecular Medicine Reports, 3, 519-525. https://doi.org/10.3892/mmr_00000291
MLA
Han, Y. H., Moon, H. J., You, B. R., Yang, Y. M., Kim, S. Z., Kim, S. H., Park, W. H."The MEK inhibitor PD98059 attenuates growth inhibition and death in gallic acid-treated Calu-6 lung cancer cells by preventing glutathione depletion". Molecular Medicine Reports 3.3 (2010): 519-525.
Chicago
Han, Y. H., Moon, H. J., You, B. R., Yang, Y. M., Kim, S. Z., Kim, S. H., Park, W. H."The MEK inhibitor PD98059 attenuates growth inhibition and death in gallic acid-treated Calu-6 lung cancer cells by preventing glutathione depletion". Molecular Medicine Reports 3, no. 3 (2010): 519-525. https://doi.org/10.3892/mmr_00000291