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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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July-August 2010 Volume 3 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

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Article

B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors

  • Authors:
    • Dominique Zehnpfennig
    • Helmut Deissler
    • Axel Polack
    • Daniel Herr
    • Georg W. Bornkamm
    • Christian Kurzeder

  • View Affiliations / Copyright

    Affiliations: Department of Child and Adolescent Psychiatry/Psychotherapy, University of Ulm Medical School, 89075 Ulm, Germany
  • Pages: 689-692
    |
    Published online on: July 1, 2010
       https://doi.org/10.3892/mmr_00000318
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Abstract

Retroviral vectors have been considered the most promising vehicles for the transfer of therapeutic genes into cells of the hematopoietic system. In clinical studies, however, cases of leukemoid clonogenic expansion of the transduced cells in vivo caused by semirandom integration of the foreign DNA in the genome have changed the focus to other types of vectors. In addition to their superior safety profile, a higher packaging capacity might be an advantage of non-viral vectors in certain applications. Prolonged transgene expression of non-viral vectors can be achieved by inclusion of Epstein-Barr virus (EBV)-derived elements mediating episomal replication and retention. Furthermore, a variety of cis acting elements have been explored in an attempt to enhance gene transfer efficiency. Our study confirmed that prolonged transgene expression can be achieved in B-lymphoid cells with EBV-derived vectors containing the EBV latent gene EBNA-1 and the EBV latent origin of replication oriP. In addition, we demonstrated that the inclusion of enhancer elements of the immunoglobulin κ light chain (Ei and E3') associated with its matrix attachment region (MAR) resulted in a 10-fold increase in transgene expression in B-lymphoid cells. It can be concluded that these elements are generally useful modules for improving the efficiencies of non-viral vectors in the B-lymphoid lineage.

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Copy and paste a formatted citation
Spandidos Publications style
Zehnpfennig D, Deissler H, Polack A, Herr D, Bornkamm GW and Kurzeder C: B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors . Mol Med Rep 3: 689-692, 2010.
APA
Zehnpfennig, D., Deissler, H., Polack, A., Herr, D., Bornkamm, G.W., & Kurzeder, C. (2010). B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors . Molecular Medicine Reports, 3, 689-692. https://doi.org/10.3892/mmr_00000318
MLA
Zehnpfennig, D., Deissler, H., Polack, A., Herr, D., Bornkamm, G. W., Kurzeder, C."B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors ". Molecular Medicine Reports 3.4 (2010): 689-692.
Chicago
Zehnpfennig, D., Deissler, H., Polack, A., Herr, D., Bornkamm, G. W., Kurzeder, C."B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors ". Molecular Medicine Reports 3, no. 4 (2010): 689-692. https://doi.org/10.3892/mmr_00000318
Copy and paste a formatted citation
x
Spandidos Publications style
Zehnpfennig D, Deissler H, Polack A, Herr D, Bornkamm GW and Kurzeder C: B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors . Mol Med Rep 3: 689-692, 2010.
APA
Zehnpfennig, D., Deissler, H., Polack, A., Herr, D., Bornkamm, G.W., & Kurzeder, C. (2010). B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors . Molecular Medicine Reports, 3, 689-692. https://doi.org/10.3892/mmr_00000318
MLA
Zehnpfennig, D., Deissler, H., Polack, A., Herr, D., Bornkamm, G. W., Kurzeder, C."B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors ". Molecular Medicine Reports 3.4 (2010): 689-692.
Chicago
Zehnpfennig, D., Deissler, H., Polack, A., Herr, D., Bornkamm, G. W., Kurzeder, C."B-cell-specific elements enhance sustained gene expression mediated by self-replicating extrachromosomal vectors ". Molecular Medicine Reports 3, no. 4 (2010): 689-692. https://doi.org/10.3892/mmr_00000318
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