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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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July-August 2010 Volume 3 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity

  • Authors:
    • Min Xu
    • Jun Gao
    • Yi Qi Du
    • Dao-Jian Gao
    • Yu Qi Zhang
    • Zhao Shen Li
    • You Li Zhang
    • Yan Fang Gong
    • Ping Xu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, P.R. China
  • Pages: 699-704
    |
    Published online on: July 1, 2010
       https://doi.org/10.3892/mmr_00000320
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Abstract

Aberrant methylation leads to epigenetic changes in human genes that may cause carcinogenesis. DNA methyltransferase 1 (DNMT1) plays an important role in maintaining DNA methylation patterns during genomic DNA replication. To understand the role of this protein in pancreatic cancer cell growth and apoptosis, small interfering RNA (siRNA) oligonucleotides were used to knockdown DNMT1 expression in pancreatic cancer PaTu8988 cells. We found that the DNMT1 siRNA markedly decreased DNMT1 expression and total DNA methyltransferase activity in the cells. Upon the inhibition of DNMT1 expression, the proliferation of the tumor cells was inhibited. Tumor cell growth was arrested in the S-phase of the cell cycle and cells underwent apoptosis. The expression of p21 was up-regulated and the ratio of Bax/Bcl-2 expression was increased after DNMT1 knockdown in PaTu8988 cells. Furthermore, DNMT1 siRNA caused demethylation of the tumor suppressor gene hMLH1, resulting in its re-expression in PaTu8988 cells. The results of this study suggest that DNMT1 siRNA oligonucleotides are candidates for further evaluation as therapeutic tools for the clinical control of pancreatic cancer.

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Copy and paste a formatted citation
Spandidos Publications style
Xu M, Gao J, Du YQ, Gao D, Zhang YQ, Li ZS, Zhang YL, Gong YF and Xu P: Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity . Mol Med Rep 3: 699-704, 2010.
APA
Xu, M., Gao, J., Du, Y.Q., Gao, D., Zhang, Y.Q., Li, Z.S. ... Xu, P. (2010). Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity . Molecular Medicine Reports, 3, 699-704. https://doi.org/10.3892/mmr_00000320
MLA
Xu, M., Gao, J., Du, Y. Q., Gao, D., Zhang, Y. Q., Li, Z. S., Zhang, Y. L., Gong, Y. F., Xu, P."Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity ". Molecular Medicine Reports 3.4 (2010): 699-704.
Chicago
Xu, M., Gao, J., Du, Y. Q., Gao, D., Zhang, Y. Q., Li, Z. S., Zhang, Y. L., Gong, Y. F., Xu, P."Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity ". Molecular Medicine Reports 3, no. 4 (2010): 699-704. https://doi.org/10.3892/mmr_00000320
Copy and paste a formatted citation
x
Spandidos Publications style
Xu M, Gao J, Du YQ, Gao D, Zhang YQ, Li ZS, Zhang YL, Gong YF and Xu P: Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity . Mol Med Rep 3: 699-704, 2010.
APA
Xu, M., Gao, J., Du, Y.Q., Gao, D., Zhang, Y.Q., Li, Z.S. ... Xu, P. (2010). Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity . Molecular Medicine Reports, 3, 699-704. https://doi.org/10.3892/mmr_00000320
MLA
Xu, M., Gao, J., Du, Y. Q., Gao, D., Zhang, Y. Q., Li, Z. S., Zhang, Y. L., Gong, Y. F., Xu, P."Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity ". Molecular Medicine Reports 3.4 (2010): 699-704.
Chicago
Xu, M., Gao, J., Du, Y. Q., Gao, D., Zhang, Y. Q., Li, Z. S., Zhang, Y. L., Gong, Y. F., Xu, P."Reduction of pancreatic cancer cell viability and induction of apoptosis mediated by siRNA targeting DNMT1 through suppression of total DNA methyltransferase activity ". Molecular Medicine Reports 3, no. 4 (2010): 699-704. https://doi.org/10.3892/mmr_00000320
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