Open Access

Cytokine-induced killer cells co-cultured with dendritic cells loaded with the protein lysate produced by radiofrequency ablation induce a specific antitumor response

  • Authors:
    • Chan‑Chan Shan
    • Liang‑Rong Shi
    • Mei‑Qian Ding
    • Yi‑Bei Zhu
    • Xiao‑Dong Li
    • Bin Xu
    • Jing‑Ting Jiang
    • Chang‑Ping Wu
  • View Affiliations

  • Published online on: February 17, 2015     https://doi.org/10.3892/ol.2015.2977
  • Pages: 1549-1556
  • Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Radiofrequency ablation (RFA) causes coagulative necrosis of tumor tissue and the production of local tumor protein debris. These fragments of tumor protein debris contain a large number of various antigens, which can stimulate a specific cellular immune response. In the present study, dendritic cells (DCs) were loaded with tumor protein lysate antigens that were produced in situ by RFA, and were used to treat murine colon carcinoma in combination with cytokine‑induced killer (CIK) cells. Subsequent to the treatment of murine colon carcinoma by RFA, the in situ supernatant of tumor lysis was collected and the DCs were loaded with the lysate antigen to generate Ag‑DCs. CIK cells induced from the spleen cells of mice were co‑cultured with Ag‑DCs to generate Ag‑DC‑CIK cells. The results revealed that the Ag‑DC‑CIK cells exhibited strong antitumor activity in vitro and in vivo. The morphology and immunophenotypes of these cells were determined using microscopy and flow cytometry, respectively. The cytotoxic activity of Ag-DC-CIK cells was determined using a CCK-8 assay. To establish a mouse model, mice were randomized into Ag-DC-CIK, DC-CIK, CIK and PBS control groups and monitored for tumor growth and survival time. ANOVA was used to compare the trends in the three groups for implanted tumor volumes. The log-rank test was used to compare the survival time. The present findings indicated that DCs loaded with the protein lysate antigens of tumors, produced in situ by RFA, combined with CIK cells may be a novel strategy for cancer treatment.

Related Articles

Journal Cover

April-2015
Volume 9 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Shan CC, Shi LR, Ding MQ, Zhu YB, Li XD, Xu B, Jiang JT and Wu CP: Cytokine-induced killer cells co-cultured with dendritic cells loaded with the protein lysate produced by radiofrequency ablation induce a specific antitumor response. Oncol Lett 9: 1549-1556, 2015.
APA
Shan, C., Shi, L., Ding, M., Zhu, Y., Li, X., Xu, B. ... Wu, C. (2015). Cytokine-induced killer cells co-cultured with dendritic cells loaded with the protein lysate produced by radiofrequency ablation induce a specific antitumor response. Oncology Letters, 9, 1549-1556. https://doi.org/10.3892/ol.2015.2977
MLA
Shan, C., Shi, L., Ding, M., Zhu, Y., Li, X., Xu, B., Jiang, J., Wu, C."Cytokine-induced killer cells co-cultured with dendritic cells loaded with the protein lysate produced by radiofrequency ablation induce a specific antitumor response". Oncology Letters 9.4 (2015): 1549-1556.
Chicago
Shan, C., Shi, L., Ding, M., Zhu, Y., Li, X., Xu, B., Jiang, J., Wu, C."Cytokine-induced killer cells co-cultured with dendritic cells loaded with the protein lysate produced by radiofrequency ablation induce a specific antitumor response". Oncology Letters 9, no. 4 (2015): 1549-1556. https://doi.org/10.3892/ol.2015.2977