Open Access

Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA‑654‑3p

  • Authors:
    • Rongjun Gao
    • Hui Ye
    • Qingjun Gao
    • Nanpeng Wang
    • Yan Zhou
    • Haisong Duan
  • View Affiliations

  • Published online on: July 19, 2021     https://doi.org/10.3892/ol.2021.12934
  • Article Number: 673
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Thyroid cancer is derived from follicular or thyroid cells and has become the most prevalent malignant tumor of endocrine organs, with increased morbidity and mortality. Circular RNAs (circRNAs) are used as prognostic and predictive markers for different types of cancer. However, the role of circRNA_0000285 in thyroid cancer and its potential molecular mechanism remain unclear. The present study aimed to investigate the roles and underlying molecular mechanism of circRNA_0000285 in thyroid cancer to identify novel treatments for this disease. The target binding site of circRNA_0000285 and microRNA‑654‑3p (miR‑654‑3p) were predicted and confirmed via the dual‑luciferase reporter and RNA immunoprecipitation (RIP) assays. Thyroid cancer cell viability and apoptosis were determined via the MTT assay and flow cytometric analysis, respectively, whereas the expression levels of circRNA_0000285 and miR‑654‑3p were determined via reverse transcription‑quantitative PCR analysis. In addition, the protein expression levels of the apoptosis‑associated proteins, Bax and B‑cell lymphoma 2 (Bcl‑2), were detected via western blotting. The results of the dual‑luciferase reporter and RIP assays demonstrated that miR‑654‑3p directly targeted circRNA_0000285. The expression levels of circRNA_0000285 and miR‑654‑3p in thyroid cancer cells (TPC‑1 and FTC133) were upregulated and downregulated, respectively. Knockdown of circRNA_0000285 via small interfering (si)RNA inhibited circRNA_0000285 levels and increased miR‑654‑3p levels. In addition, miR‑654‑3p expression decreased following transfection with miR‑654‑3p inhibitor. Functional experiments demonstrated that circRNA_0000285‑siRNA decreased thyroid cancer cell proliferation, promoted cell apoptosis, enhanced Bax expression and suppressed Bcl‑2 expression. All these effects were reversed following transfection with miR‑654‑3p inhibitor. Taken together, the results of the present study suggest that circRNA_0000285 plays a vital role in thyroid cancer progression by regulating miR‑654‑3p, which provides a potential therapeutic target for this disease.

Related Articles

Journal Cover

September-2021
Volume 22 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Gao R, Ye H, Gao Q, Wang N, Zhou Y and Duan H: Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA‑654‑3p. Oncol Lett 22: 673, 2021
APA
Gao, R., Ye, H., Gao, Q., Wang, N., Zhou, Y., & Duan, H. (2021). Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA‑654‑3p. Oncology Letters, 22, 673. https://doi.org/10.3892/ol.2021.12934
MLA
Gao, R., Ye, H., Gao, Q., Wang, N., Zhou, Y., Duan, H."Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA‑654‑3p". Oncology Letters 22.3 (2021): 673.
Chicago
Gao, R., Ye, H., Gao, Q., Wang, N., Zhou, Y., Duan, H."Inhibition of circular RNA_0000285 prevents cell proliferation and induces apoptosis in thyroid cancer by sponging microRNA‑654‑3p". Oncology Letters 22, no. 3 (2021): 673. https://doi.org/10.3892/ol.2021.12934