Open Access

Identification and verification of the role of crucial genes through which methionine restriction inhibits the progression of colon cancer cells

  • Authors:
    • Liqiang Zhou
    • Zhiqing Chen
    • Chuan Liu
  • View Affiliations

  • Published online on: June 22, 2022     https://doi.org/10.3892/ol.2022.13394
  • Article Number: 274
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Studies have shown that methionine restriction (MR) can inhibit tumor progression, but its mechanism in colon cancer is unknown. Through DESeq2 and Edge analysis of the GSE72131 and GSE103602 datasets, 649 co‑upregulated and 532 co‑downregulated genes affected by MR were identified, respectively. Enrichment analysis showed that these genes were closely associated with tumor progression. Combined with the differentially expressed genes of colon cancer in The Cancer Genome Atlas database, MR affected 330 dysregulated genes in colon cancer. On this basis, a transcriptional regulatory and competing endogenous RNA network was established and F transcription factor 1 and microRNA 17‑92a‑1 Cluster Host Gene were identified as a key transcription factor and long non‑coding RNA, respectively. In addition, four genes (FA Complementation Group I, Holliday Junction Recognition Protein, Karyopherin Subunit Alpha 2 and Kinesin Family Member 15) were identified by analyzing the relationship between dysregulated genes and overall survival. Finally, western blotting, reverse transcription‑quantitative PCR, Transwell and other in vitro experiments verified that MR inhibits HCT116 colon cancer cell proliferation, metastasis and invasion, induces apoptosis and downregulates 6 hub genes. Collectively, the present study identified potential targets for MR to inhibit colon cancer progression and contributed to the clinical application of MR.

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August-2022
Volume 24 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhou L, Chen Z and Liu C: Identification and verification of the role of crucial genes through which methionine restriction inhibits the progression of colon cancer cells. Oncol Lett 24: 274, 2022
APA
Zhou, L., Chen, Z., & Liu, C. (2022). Identification and verification of the role of crucial genes through which methionine restriction inhibits the progression of colon cancer cells. Oncology Letters, 24, 274. https://doi.org/10.3892/ol.2022.13394
MLA
Zhou, L., Chen, Z., Liu, C."Identification and verification of the role of crucial genes through which methionine restriction inhibits the progression of colon cancer cells". Oncology Letters 24.2 (2022): 274.
Chicago
Zhou, L., Chen, Z., Liu, C."Identification and verification of the role of crucial genes through which methionine restriction inhibits the progression of colon cancer cells". Oncology Letters 24, no. 2 (2022): 274. https://doi.org/10.3892/ol.2022.13394