TRANSFORMATION OF PRIMARY CHICK-EMBRYO FIBROBLASTS BY VIRALLY-EXPRESSED C-JUN REQUIRES NO MUTATION IN THE PROTEIN WHEN A SUFFICIENT LEVEL OF C-JUN PROTEIN IS REACHED

  • Authors:
    • N LAVISTA
    • F PIU
    • L LOISEAU
    • G BRUN
    • M CASTELLAZZI
  • View Affiliations

  • Published online on: January 1, 1994     https://doi.org/10.3892/or.1.1.185
  • Pages: 185-190
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Abstract

Proto-oncogene c-jun transforms primary chick embryo fibroblasts as a single gene when expressed from the replication competent retrovirus R (or RCAS). We have investigated whether transformation is caused by accumulating mutations within the proto-oncogene sequence during the retrotranscription process. c-jun coding sequences carried by three independently isolated R-cJUN viruses have been cloned and entirely sequenced. Two of these-were shown to carry no mutation demonstrating that deregulated expression of a normal c-Jun product is sufficient for transformation. On the contrary when expressed from non replicative Rneo vector lacking gag and pol sequences, the avian c-jun no longer transformed. This difference is correlated with weaker c-Jun accumulation in the second case. When expressed from the same Rneo vector, low level of v-Jun or of the heterologous mouse c-Jun still transform. Taken together, these data strongly suggest that a threshold level of c-Jun product is required for transformation and that this critical level can be lowered by mutations in c-jun or by the synthesis of an heterologous protein.

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January 1994
Volume 1 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
LAVISTA N, PIU F, LOISEAU L, BRUN G and CASTELLAZZI M: TRANSFORMATION OF PRIMARY CHICK-EMBRYO FIBROBLASTS BY VIRALLY-EXPRESSED C-JUN REQUIRES NO MUTATION IN THE PROTEIN WHEN A SUFFICIENT LEVEL OF C-JUN PROTEIN IS REACHED. Oncol Rep 1: 185-190, 1994
APA
LAVISTA, N., PIU, F., LOISEAU, L., BRUN, G., & CASTELLAZZI, M. (1994). TRANSFORMATION OF PRIMARY CHICK-EMBRYO FIBROBLASTS BY VIRALLY-EXPRESSED C-JUN REQUIRES NO MUTATION IN THE PROTEIN WHEN A SUFFICIENT LEVEL OF C-JUN PROTEIN IS REACHED. Oncology Reports, 1, 185-190. https://doi.org/10.3892/or.1.1.185
MLA
LAVISTA, N., PIU, F., LOISEAU, L., BRUN, G., CASTELLAZZI, M."TRANSFORMATION OF PRIMARY CHICK-EMBRYO FIBROBLASTS BY VIRALLY-EXPRESSED C-JUN REQUIRES NO MUTATION IN THE PROTEIN WHEN A SUFFICIENT LEVEL OF C-JUN PROTEIN IS REACHED". Oncology Reports 1.1 (1994): 185-190.
Chicago
LAVISTA, N., PIU, F., LOISEAU, L., BRUN, G., CASTELLAZZI, M."TRANSFORMATION OF PRIMARY CHICK-EMBRYO FIBROBLASTS BY VIRALLY-EXPRESSED C-JUN REQUIRES NO MUTATION IN THE PROTEIN WHEN A SUFFICIENT LEVEL OF C-JUN PROTEIN IS REACHED". Oncology Reports 1, no. 1 (1994): 185-190. https://doi.org/10.3892/or.1.1.185