O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY IN TISSUES OF MICE TREATED WITH ANTISCHISTOSOMAL AGENTS

  • Authors:
    • AF BADAWI
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  • Published online on: September 1, 1994     https://doi.org/10.3892/or.1.5.1023
  • Pages: 1023-1027
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Abstract

The activity of O-6-alkylguanine-DNA alkyl transferase (ATase), the enzyme responsible for repairing promutagenic methylation damage in DNA, was measured at various time intervals in tissue extracts of mice administered (in vivo) a single therapeutic dose of the antischistosomal agents hycanthone, oxaminiquine and metrifonate. In control animals, liver contained the highest levels of ATase activity (15.8+/-1.8 fmole ATase/mu g DNA) followed by spleen (11.0+/-1.7 fmole/mu g DNA), intestine (2.3+/-0.3 fmole/mu g DNA) and bladder (0.22+/-0.04 fmole/mu g DNA). With hycanthone, ATase activity was reduced by 6 and 24 h post treatment to 74% and 27% below the control value, respectively. Bladder exhibited a 25% inactivation in the ATase level at 6 h time point. Spleen and bladder did not show any alteration in the ATase activity. In animals administered oxaminiquine, liver and bladder had a near identical pattern to that observed for hycanthone. Spleen and intestine, however, revealed activation in ATase by 50% and 42%, respectively after 6 h of treatment. This activation was also observed in the bladder of metrifonate-treated mice. In a previous study (Badawi et al, Cancer Lett 75: 167, 1993), DNA-alkylation damage (O-6-methyldeoxyguanosine; O-6-MedG) was evaluated in these tissues and there was an inverse correlation between the levels of methylation damage and ATase activity in liver (r=-0.85, p<0.01), intestine (r=-0.62, p<0.01) and bladder (r=-0.59, p<0.05).

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September 1994
Volume 1 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
BADAWI A: O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY IN TISSUES OF MICE TREATED WITH ANTISCHISTOSOMAL AGENTS. Oncol Rep 1: 1023-1027, 1994
APA
BADAWI, A. (1994). O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY IN TISSUES OF MICE TREATED WITH ANTISCHISTOSOMAL AGENTS. Oncology Reports, 1, 1023-1027. https://doi.org/10.3892/or.1.5.1023
MLA
BADAWI, A."O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY IN TISSUES OF MICE TREATED WITH ANTISCHISTOSOMAL AGENTS". Oncology Reports 1.5 (1994): 1023-1027.
Chicago
BADAWI, A."O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE ACTIVITY IN TISSUES OF MICE TREATED WITH ANTISCHISTOSOMAL AGENTS". Oncology Reports 1, no. 5 (1994): 1023-1027. https://doi.org/10.3892/or.1.5.1023