Effect of retinoblastoma tumor suppressor gene expression on chemosensitivity of human osteosarcoma cell lines

  • Authors:
    • Koji Iida
    • Tsutomu Nobori
    • Akihiko Matsumine
    • Akihiko Isaka
    • Masashi Seto
    • Taizou Shiraishi
    • Atsumasa Uchida
  • View Affiliations

  • Published online on: November 1, 2003     https://doi.org/10.3892/or.10.6.1961
  • Pages: 1961-1965
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We examined the effects of inactivation of the RB gene on chemosensitivity of human osteosarcoma cell lines, using the MTT assay and calculating the inhibition index. Although the human osteosarcoma cell lines HOS and MG63 have a wild-type RB gene, SaOS-2 and OSrb (established from retinoblastoma patient) have no active RB gene. We used these 4 cell lines in growth inhibition assays for doxorubicin, cisplatin and methotrexate, and assessed the chemosensitivity. In the growth inhibition assay for methotrexate, cell lines lacking an active RB gene were more resistant than cell lines with an active RB gene.

Related Articles

Journal Cover

November-December 2003
Volume 10 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Iida K, Nobori T, Matsumine A, Isaka A, Seto M, Shiraishi T and Uchida A: Effect of retinoblastoma tumor suppressor gene expression on chemosensitivity of human osteosarcoma cell lines. Oncol Rep 10: 1961-1965, 2003
APA
Iida, K., Nobori, T., Matsumine, A., Isaka, A., Seto, M., Shiraishi, T., & Uchida, A. (2003). Effect of retinoblastoma tumor suppressor gene expression on chemosensitivity of human osteosarcoma cell lines. Oncology Reports, 10, 1961-1965. https://doi.org/10.3892/or.10.6.1961
MLA
Iida, K., Nobori, T., Matsumine, A., Isaka, A., Seto, M., Shiraishi, T., Uchida, A."Effect of retinoblastoma tumor suppressor gene expression on chemosensitivity of human osteosarcoma cell lines". Oncology Reports 10.6 (2003): 1961-1965.
Chicago
Iida, K., Nobori, T., Matsumine, A., Isaka, A., Seto, M., Shiraishi, T., Uchida, A."Effect of retinoblastoma tumor suppressor gene expression on chemosensitivity of human osteosarcoma cell lines". Oncology Reports 10, no. 6 (2003): 1961-1965. https://doi.org/10.3892/or.10.6.1961