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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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February 2004 Volume 11 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP

  • Authors:
    • Hideaki Dote
    • Kazunori Tsukuda
    • Shinichi Toyooka
    • Masaaki Yano
    • Harvey I. Pass
    • Nobuyoshi Shimizu

  • View Affiliations / Copyright

    Affiliations: Department of Cancer and Thoracic Surgery, Graduate School of Medicine and Dentistry, Okayama University, Okayama 700-8558, Japan
  • Pages: 361-363
    |
    Published online on: February 1, 2004
       https://doi.org/10.3892/or.11.2.361
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Abstract

BRAF encodes a RAS-regulated serine/threonine kinase that mediates the pathway for cell growth and malignant transformation. Point mutations of BRAF were reported recently in 66% of melanomas, over 30% of thyroid papillary and low-grade ovarian cancers, and a smaller percentage of other human cancers. Mutations in malignant cells were reported to occur only in exons 11 and 15. Among these mutations, BRAF V599E is most frequent and proved to invert its transcript to the dominant active form. To exclude the interference of co-existing normal cells in clinical samples, we developed a new enriched PCR-RFLP assay for detecting mutations of BRAF codon 599 mutation. The sensitivity of this assay was examined to find that one mutant allele among 102 wild-type alleles could be detected. We applied this method for 53 cases of primary malignant pleural mesotheliomas (MPMs) and 6 cell lines and found no mutations in these samples. Our results demonstrate that the developed enriched PCR-RFLP is a sensitive assay to detect BRAF codon 599 mutation. However, it may be a rare type of mutation in MPMs. Our new assay is useful and can be applied for screening of BRAF codon 599 mutation in various kinds of clinical samples.

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Copy and paste a formatted citation
Spandidos Publications style
Dote H, Tsukuda K, Toyooka S, Yano M, Pass HI and Shimizu N: Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP. Oncol Rep 11: 361-363, 2004.
APA
Dote, H., Tsukuda, K., Toyooka, S., Yano, M., Pass, H.I., & Shimizu, N. (2004). Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP. Oncology Reports, 11, 361-363. https://doi.org/10.3892/or.11.2.361
MLA
Dote, H., Tsukuda, K., Toyooka, S., Yano, M., Pass, H. I., Shimizu, N."Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP". Oncology Reports 11.2 (2004): 361-363.
Chicago
Dote, H., Tsukuda, K., Toyooka, S., Yano, M., Pass, H. I., Shimizu, N."Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP". Oncology Reports 11, no. 2 (2004): 361-363. https://doi.org/10.3892/or.11.2.361
Copy and paste a formatted citation
x
Spandidos Publications style
Dote H, Tsukuda K, Toyooka S, Yano M, Pass HI and Shimizu N: Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP. Oncol Rep 11: 361-363, 2004.
APA
Dote, H., Tsukuda, K., Toyooka, S., Yano, M., Pass, H.I., & Shimizu, N. (2004). Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP. Oncology Reports, 11, 361-363. https://doi.org/10.3892/or.11.2.361
MLA
Dote, H., Tsukuda, K., Toyooka, S., Yano, M., Pass, H. I., Shimizu, N."Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP". Oncology Reports 11.2 (2004): 361-363.
Chicago
Dote, H., Tsukuda, K., Toyooka, S., Yano, M., Pass, H. I., Shimizu, N."Mutation analysis of the BRAF codon 599 in malignant pleural mesothelioma by enriched PCR-RFLP". Oncology Reports 11, no. 2 (2004): 361-363. https://doi.org/10.3892/or.11.2.361
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