Enhancement of cytotoxicity of NK cells by D-Fraction, a polysaccharide from Grifola frondosa

  • Authors:
    • Noriko Kodama
    • Akihiro Asakawa
    • Akio Inui
    • Yuki Masuda
    • Hiroaki Nanba
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/or.13.3.497
  • Pages:497-502
0

Abstract

In innate immunity, activated natural killer (NK) cells attack and damage pathogens such as bacteria and virus without restriction by the MHC antigen. NK cells activated by IL-12 have been reported to recognize and kill tumor cells in perforin-mediated apoptosis. We have reported that D-Fraction, a polysaccharide extracted from the maitake mushroom (Grifola frondosa), activates macrophages, dendritic cells, and T cells and inhibits the growth of tumor cells. However, the effects of D-Fraction on NK cell function in the innate immune response are not well known. In the present study, we administered D-Fraction to MM-46 mammary tumor-bearing C3H/HeJ mice intraperitoneally for 3 consecutive days and investigated its effects on the activation and cytotoxicity of NK cells. D-Fraction significantly enhanced the cytotoxicity against NK-sensitive YAC-1 cells and the expression of CD223 on NK cells. D-Fraction also increased the expression of CD86 on macrophages. In addition, the levels of IL-12 in the culture supernatant of whole spleen cells and in serum increased, compared with the control corresponding to an increase in expression of IL-12 receptor βI on NK cells. These results suggest that D-Fraction enhances the cytotoxicity of NK cells through the production of IL-12 by macrophages activated by D-Fraction.

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March 2005
Volume 13 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

2015 Impact Factor: 2.486
Ranked #31/211 Oncology
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APA
Kodama, N., Asakawa, A., Inui, A., Masuda, Y., & Nanba, H. (2005). Enhancement of cytotoxicity of NK cells by D-Fraction, a polysaccharide from Grifola frondosa. Oncology Reports, 13, 497-502. https://doi.org/10.3892/or.13.3.497
MLA
Kodama, N., Asakawa, A., Inui, A., Masuda, Y., Nanba, H."Enhancement of cytotoxicity of NK cells by D-Fraction, a polysaccharide from Grifola frondosa". Oncology Reports 13.3 (2005): 497-502.
Chicago
Kodama, N., Asakawa, A., Inui, A., Masuda, Y., Nanba, H."Enhancement of cytotoxicity of NK cells by D-Fraction, a polysaccharide from Grifola frondosa". Oncology Reports 13, no. 3 (2005): 497-502. https://doi.org/10.3892/or.13.3.497