Differential expression of cytokines, chemokines and their receptors in follicular lymphoma and reactive follicular hyperplasia: Assessment by complementary DNA microarray

  • Authors:
    • Akira Fujii
    • Koichi Oshima
    • Makoto Hamasaki
    • Hidetsuna Utsunomiya
    • Masatoshi Okazaki
    • Yoshitoyo Kagami
    • Masao Seto
    • Masahiro Kikuchi
  • View Affiliations

  • Published online on: May 1, 2005     https://doi.org/10.3892/or.13.5.819
  • Pages: 819-824
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Abstract

Follicular lymphoma (FL) is pathologically categorised as a low-grade B-cell lymphoma and histopathologically shows follicular proliferation of neoplastic B cells. In the neoplastic follicles of FL, the presence of T cells, macrophages and follicular dendritic cells (FDCs) suggests that these cells may promote a favourable environment for the growth of FL cells. Because FL cells are generally associated with FDCs, FDCs may be considered an important source of cytokines and chemokines. FDCs form the framework for germinal centres and also provide networks for nodules of FL. To evaluate the gene expression in neoplastic follicles of FL and reactive follicles of reactive follicular hyperplasia (RFH), we performed gene expression profiling of FL (n=5) and RFH (n=5) using complementary DNA (cDNA) microarray of cytokines/chemokines and their receptors. FL and RFH exhibited a diffuse down-regulated profile compared with normal peripheral blood cells, which were used as controls, although some genes displayed up-regulated profiles. Hierarchical clustering analysis separated FL and RFH into two distinct groups based on their gene expression profiles. FL cases exhibited significantly higher expression of interleukin 3 receptor α (IL-3Rα) than RFH. Immunohistochemically, neoplastic follicles of FL frequently expressed IL-3Rα, especially in FDCs, but not in FL cells. However, IL-3Rα expression was rare or weak in the reactive follicles of RFH. These findings suggest the importance of the micro-environment for FL cell growth. Further studies of cDNA microarray should provide new insight into the molecular pathology of FL and may allow the design of improved therapies.

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May 2005
Volume 13 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Fujii A, Oshima K, Hamasaki M, Utsunomiya H, Okazaki M, Kagami Y, Seto M and Kikuchi M: Differential expression of cytokines, chemokines and their receptors in follicular lymphoma and reactive follicular hyperplasia: Assessment by complementary DNA microarray. Oncol Rep 13: 819-824, 2005
APA
Fujii, A., Oshima, K., Hamasaki, M., Utsunomiya, H., Okazaki, M., Kagami, Y. ... Kikuchi, M. (2005). Differential expression of cytokines, chemokines and their receptors in follicular lymphoma and reactive follicular hyperplasia: Assessment by complementary DNA microarray. Oncology Reports, 13, 819-824. https://doi.org/10.3892/or.13.5.819
MLA
Fujii, A., Oshima, K., Hamasaki, M., Utsunomiya, H., Okazaki, M., Kagami, Y., Seto, M., Kikuchi, M."Differential expression of cytokines, chemokines and their receptors in follicular lymphoma and reactive follicular hyperplasia: Assessment by complementary DNA microarray". Oncology Reports 13.5 (2005): 819-824.
Chicago
Fujii, A., Oshima, K., Hamasaki, M., Utsunomiya, H., Okazaki, M., Kagami, Y., Seto, M., Kikuchi, M."Differential expression of cytokines, chemokines and their receptors in follicular lymphoma and reactive follicular hyperplasia: Assessment by complementary DNA microarray". Oncology Reports 13, no. 5 (2005): 819-824. https://doi.org/10.3892/or.13.5.819