The association of Id-1, MIF and GSTpi with acquired drug resistance in hormone independent prostate cancer cells

  • Authors:
    • Jau-Chen Lin
    • Sun-Yran Chang
    • Dar-Shih Hsieh
    • Chi-Feng Lee
    • Dah-Shyong Yu
  • View Affiliations

  • Published online on: May 1, 2005     https://doi.org/10.3892/or.13.5.983
  • Pages: 983-988
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Most patients with prostate cancer respond to androgen ablation therapy, but the tumor eventually progresses to an androgen-independent stage with multiple anti-cancer drug resistance. Novel therapeutic strategies for hormone independent prostate cancer need to be developed. The genes for Id-1, MIF and GSTpi were up-regulated in drug resistant cells of hormone independent prostate cancer cells using cDNA microarray gene expression analysis. In this study we aimed to investigate whether constitutive overexpression of these candidate genes in cells can affect cellular resistance to chemotherapeutic agents. The mRNA expression was determined by reverse transcriptase-polymerase chain reaction, and the Id-1, MIF and GSTpi protein contents in these cell lines were measured by Western blotting. Susceptibility of the cells to chemotherapeutic agents including doxorubicin, paclitaxel and cyclophosphamide was determined by microculture tetrazolium bromide assay. The analysis of apoptosis was assessed by annexin V assay. The cytotoxity assay results demonstrated that cells overexpressing the Id-1 gene increased in their resistance to doxorubicin, paclitaxel and cyclophosphamide. MIF expression can drive cells to increase in their resistance to paclitaxel, and GSTpi expression confers drug resistance to doxorubicin and cyclophosphamide. The induction of mdr1 gene expression was noted in up-regulation of MIF and GSTpi. Our findings suggest that overproduction of the Id-1, MIF and GSTpi proteins and coinduction of mdr-1 play an important role in the development of acquired drug resistance to various drugs of prostate cancer cells.

Related Articles

Journal Cover

May 2005
Volume 13 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lin J, Chang S, Hsieh D, Lee C and Yu D: The association of Id-1, MIF and GSTpi with acquired drug resistance in hormone independent prostate cancer cells. Oncol Rep 13: 983-988, 2005
APA
Lin, J., Chang, S., Hsieh, D., Lee, C., & Yu, D. (2005). The association of Id-1, MIF and GSTpi with acquired drug resistance in hormone independent prostate cancer cells. Oncology Reports, 13, 983-988. https://doi.org/10.3892/or.13.5.983
MLA
Lin, J., Chang, S., Hsieh, D., Lee, C., Yu, D."The association of Id-1, MIF and GSTpi with acquired drug resistance in hormone independent prostate cancer cells". Oncology Reports 13.5 (2005): 983-988.
Chicago
Lin, J., Chang, S., Hsieh, D., Lee, C., Yu, D."The association of Id-1, MIF and GSTpi with acquired drug resistance in hormone independent prostate cancer cells". Oncology Reports 13, no. 5 (2005): 983-988. https://doi.org/10.3892/or.13.5.983