Up-regulation of growth factor receptor-bound protein 10 in cervical squamous cell carcinoma

  • Authors:
    • Keiko Okino
    • Hideki Konishi
    • Daisuke Doi
    • Koichi Yoneyama
    • Yujiro Ota
    • Enjing Jin
    • Oichi Kawanami
    • Toshiyuki Takeshita
  • View Affiliations

  • Published online on: June 1, 2005     https://doi.org/10.3892/or.13.6.1069
  • Pages: 1069-1074
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Abstract

The Grb10 gene on chromosome 7p11.2-p12 belongs to a family of adapter proteins known to interact with a number of receptor tyrosine kinases, such as EGF, ErbB2/Her2, platelet-derived growth factor (PDGF), IGF-I receptors and vascular endothelial growth factor (VEGF) receptor, KDR (kinase insert domain containing receptor). In addition to receptor tyrosine kinases, Grb10 has also been found to interact with non-receptor tyrosine kinases such as Tec and Bcr-Abl, other cellular signaling molecules such as Raf-1, and the mitogen-activated protein (MAP) kinase, MEK. We demonstrated increased expression of Grb10 mRNA in more than one half of primary cervical squamous cell cancers (12 of 15 cases) when compared to corresponding non-cancerous uterine squamous cell tissues. In addition, immunohistochemical staining demonstrated that the Grb10 protein was prominent in the cytoplasm of cancer cells, whereas it was unreactive in the surrounding normal cervical squamous cells. In addition, its interruption by siRNA exhibited marked cell growth inhibition. These data indicate that amplification and increased expression of the Grb10 gene may play a role in the development of a portion of human cervical squamous cell cancer.

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June 2005
Volume 13 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Okino K, Konishi H, Doi D, Yoneyama K, Ota Y, Jin E, Kawanami O and Takeshita T: Up-regulation of growth factor receptor-bound protein 10 in cervical squamous cell carcinoma. Oncol Rep 13: 1069-1074, 2005
APA
Okino, K., Konishi, H., Doi, D., Yoneyama, K., Ota, Y., Jin, E. ... Takeshita, T. (2005). Up-regulation of growth factor receptor-bound protein 10 in cervical squamous cell carcinoma. Oncology Reports, 13, 1069-1074. https://doi.org/10.3892/or.13.6.1069
MLA
Okino, K., Konishi, H., Doi, D., Yoneyama, K., Ota, Y., Jin, E., Kawanami, O., Takeshita, T."Up-regulation of growth factor receptor-bound protein 10 in cervical squamous cell carcinoma". Oncology Reports 13.6 (2005): 1069-1074.
Chicago
Okino, K., Konishi, H., Doi, D., Yoneyama, K., Ota, Y., Jin, E., Kawanami, O., Takeshita, T."Up-regulation of growth factor receptor-bound protein 10 in cervical squamous cell carcinoma". Oncology Reports 13, no. 6 (2005): 1069-1074. https://doi.org/10.3892/or.13.6.1069