Cytokine-dependent invasiveness in B16 murine melanoma cells: Role of uPA system and MMP-9

  • Authors:
    • Francesca Bianchini
    • Silvia D'Alessio
    • Gabriella Fibbi
    • Mario Del Rosso
    • Lido Calorini
  • View Affiliations

  • Published online on: March 1, 2006     https://doi.org/10.3892/or.15.3.709
  • Pages: 709-714
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Proteases are crucial for the spread of cancer cells from a primary tumor to the site of secondary growth. This study examined the ability of IFNγ and TNFα to stimulate a better invasiveness in B16 murine melanoma cells, and investigated whether this enhanced ability was related to a higher expression of protease activities, such as urokinase plasminogen activator (uPA) and its receptor (uPAR), and matrix metalloproteinases 2 and 9 (MMP-2, MMP-9). We found that murine melanoma cells enhanced their lung-colonizing potential in vivo and invasiveness through Matrigel-coated filters upon costimulation with IFNγ and TNFα; neither IFNγ nor TNFα alone, at the dose used in the experiments, was able to elicit a change in the invasive/metastatic efficiency of melanoma cells. The invasive phenotype of murine melanoma cells stimulated with IFNγ and TNFα was characterized by an enhanced uPA/uPAR and MMP-9 expression: TNFα promoted MMP-9 mRNA expression and pro-MMP-9 protein secretion, and the costimulation with IFNγ and TNFα was required to potentiate the expression of mRNA and protein for uPAR, and to induce a redistribution of uPA from the soluble to the cell body-associated form. Both monoclonal antibodies, anti-uPAR and anti-MMP-9, caused a significant reduction of invasiveness in IFNγ/TNFα-stimulated melanoma cells. These results indicate that invasiveness in B16 murine melanoma cells can be regulated in a cytokine-specific fashion and is dependent on the synergism between the uPA/uPAR system and MMP-9.

Related Articles

Journal Cover

March 2006
Volume 15 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Bianchini F, D'Alessio S, Fibbi G, Del Rosso M and Calorini L: Cytokine-dependent invasiveness in B16 murine melanoma cells: Role of uPA system and MMP-9. Oncol Rep 15: 709-714, 2006
APA
Bianchini, F., D'Alessio, S., Fibbi, G., Del Rosso, M., & Calorini, L. (2006). Cytokine-dependent invasiveness in B16 murine melanoma cells: Role of uPA system and MMP-9. Oncology Reports, 15, 709-714. https://doi.org/10.3892/or.15.3.709
MLA
Bianchini, F., D'Alessio, S., Fibbi, G., Del Rosso, M., Calorini, L."Cytokine-dependent invasiveness in B16 murine melanoma cells: Role of uPA system and MMP-9". Oncology Reports 15.3 (2006): 709-714.
Chicago
Bianchini, F., D'Alessio, S., Fibbi, G., Del Rosso, M., Calorini, L."Cytokine-dependent invasiveness in B16 murine melanoma cells: Role of uPA system and MMP-9". Oncology Reports 15, no. 3 (2006): 709-714. https://doi.org/10.3892/or.15.3.709