L-selenomethionine modulates high LET radiation-induced alterations of gene expression in cultured human thyroid cells

  • Authors:
    • Jelena Stewart
    • Jeffrey Ware
    • Paolo Fortina
    • Jim Breaux
    • Sandeep Gulati
    • Ann Kennedy
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/or.16.3.569
  • Pages: 569-574
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Abstract

L-selenomethionine (SeM) is emerging as a highly effective protective agent against radiation-induced biological effects. We have shown its protective effect on space radiation-induced death of MCF-10 cells as well as on space radiation-induced transformation of HTori-3 cells. The present study was aimed at elucidation of molecular mechanisms and cellular pathways involved in SeM-mediated radioprotection. Human thyroid epithelial cells (HTori-3 cells), in the presence or absence of SeM, were exposed to a non-toxic or a slightly toxic radiation dose from 1 GeV/n iron ions (10 cGy and 20 cGy, respectively). Total RNA was prepared and changes in gene expression were analyzed using microarray technology. Our analysis has revealed a dramatic effect of SeM on alterations of gene expression caused by space radiation. This study provides a basis for furthering our knowledge about radiation-induced molecular and cellular changes that lead to cellular transformation and death.

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September 2006
Volume 16 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Stewart J, Ware J, Fortina P, Breaux J, Gulati S and Kennedy A: L-selenomethionine modulates high LET radiation-induced alterations of gene expression in cultured human thyroid cells. Oncol Rep 16: 569-574, 2006
APA
Stewart, J., Ware, J., Fortina, P., Breaux, J., Gulati, S., & Kennedy, A. (2006). L-selenomethionine modulates high LET radiation-induced alterations of gene expression in cultured human thyroid cells. Oncology Reports, 16, 569-574. https://doi.org/10.3892/or.16.3.569
MLA
Stewart, J., Ware, J., Fortina, P., Breaux, J., Gulati, S., Kennedy, A."L-selenomethionine modulates high LET radiation-induced alterations of gene expression in cultured human thyroid cells". Oncology Reports 16.3 (2006): 569-574.
Chicago
Stewart, J., Ware, J., Fortina, P., Breaux, J., Gulati, S., Kennedy, A."L-selenomethionine modulates high LET radiation-induced alterations of gene expression in cultured human thyroid cells". Oncology Reports 16, no. 3 (2006): 569-574. https://doi.org/10.3892/or.16.3.569