Edelfosine protects precultured heart fragments against the invasion of malignant cells through altered sialylation

Van Slambrouck,S.; Steelant,W.F. A.; Lopez,M.; Recchi,M. A.; Boilly-Marer,Y.; Bruyneel,E. A.; Delannoy,P.; Verbert,A.; Van Den Eeckhout,E. G.; Deforce,D.

doi:10.3892/or.17.2.433

Edelfosine protects precultured heart fragments against the invasion of malignant cells through altered sialylation

Authors:
- S. Van Slambrouck
- W.F. A. Steelant
- M. Lopez
- M. A. Recchi
- Y. Boilly-Marer
- E. A. Bruyneel
- P. Delannoy
- A. Verbert
- E. G. Van Den Eeckhout
- D. Deforce
View Affiliations

Affiliations: Department of Chemistry, Laboratory of Biochemical and Biomedical Research, New Mexico Tech, Socorro, NM 87801, USA
Published online on: February 1, 2007 https://doi.org/10.3892/or.17.2.433
Pages: 433-439

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

1-O-octadecyl-2-O-methylglycero-3-phosphocholine (ET-18-OMe)-treated precultured heart fragments (PHF) are resistant to the invasion of malignant cells. Previous studies have demonstrated that this effect is due to the alterations of the N-linked glycoproteins in PHF after 48-h ET-18-OMe treatment. Moreover, the observed effect was still present seven days after ET-18-OMe was omitted. The present study reveals that ≈13.4% of the administered ET-18-OMe was taken up by PHF and about 75% of the initial uptake was still present after ET-18-OMe was removed. In addition, we found significant changes in the sialic acid content and sialyltransferase activities in both conditions. Overall, these results clearly demonstrate that the uptake and retention of ET-18-OMe are responsible for the resistance to the invasion of malignant cells due to the altered sialylation of the cell surface glycoproteins in PHF.