Chemopreventive effect of fermented brown rice and rice bran on 4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats

  • Authors:
    • Nguyen Khanh Long
    • Hiroki Makita
    • Tomomi Yamashita
    • Makoto Toida
    • Keizo Kato
    • Daijiro Hatakeyama
    • Toshiyuki Shibata
  • View Affiliations

  • Published online on: April 1, 2007     https://doi.org/10.3892/or.17.4.879
  • Pages: 879-885
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Abstract

The preventive effects of the dietary administration of brown rice and rice bran fermented with Aspergillus oryzae (FBRA) on oral carcinogenesis induced by 4-nitroquinoline 1-oxide (4-NQO) were investigated in male F344 rats. At 7 weeks of age, the animals were given 20 ppm 4-NQO in their drinking water for 8 weeks to induce tongue neoplasms. Groups of rats were fed diets containing 5 or 10% FBRA during the initiation or postinitiation phases of the 4-NQO-induced oral carcinogenesis. The other groups consisted of rats fed 10% FBRA or untreated rats. At the termination of the study (week 32), the incidences, multiplicities of tongue lesions (pre-neoplasms and neoplasms) and the cell proliferation activity estimated by the 5-bromodeoxyuridine (BrdU)-labeling index were compared among the groups. Feeding of 5% FBRA during the initiation phase significantly decreased the incidence (68.2 vs 36.8%; p<0.05) and multiplicity (1.05±0.84 vs 0.37±0.50; p<0.005) of the tongue carcinoma. When feeding of 10% FBRA occurred after the 4-NQO exposure, the multiplicity of tongue carcinoma was also reduced (1.05±0.84 vs 0.52±0.60; p<0.05). In addition, the dietary administration of FBRA at both doses significantly decreased the BrdU-labeling index in the oral squamous epithelium (p<0.05). Although a dose-dependent response was not observed, FBRA is effective in suppressing the development of 4-NQO-induced oral carcinogenesis by its concurrent exposure to the carcinogen. The inhibitory effect could be related to the suppression of the hyperproliferation of cells in the tongue epithelium and the radical scavenging activity of FBRA.

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April 2007
Volume 17 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Long NK, Makita H, Yamashita T, Toida M, Kato K, Hatakeyama D and Shibata T: Chemopreventive effect of fermented brown rice and rice bran on 4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats. Oncol Rep 17: 879-885, 2007.
APA
Long, N.K., Makita, H., Yamashita, T., Toida, M., Kato, K., Hatakeyama, D., & Shibata, T. (2007). Chemopreventive effect of fermented brown rice and rice bran on 4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats. Oncology Reports, 17, 879-885. https://doi.org/10.3892/or.17.4.879
MLA
Long, N. K., Makita, H., Yamashita, T., Toida, M., Kato, K., Hatakeyama, D., Shibata, T."Chemopreventive effect of fermented brown rice and rice bran on 4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats". Oncology Reports 17.4 (2007): 879-885.
Chicago
Long, N. K., Makita, H., Yamashita, T., Toida, M., Kato, K., Hatakeyama, D., Shibata, T."Chemopreventive effect of fermented brown rice and rice bran on 4-nitroquinoline 1-oxide-induced oral carcinogenesis in rats". Oncology Reports 17, no. 4 (2007): 879-885. https://doi.org/10.3892/or.17.4.879