Preparation of fully activated dendritic cells capable of priming tumor-specific cytotoxic T lymphocytes in patients with metastatic cancer using penicillin-killed streptococcus pyogenes (OK432) and anti-CD40 antibody

  • Authors:
    • Keiichi Kontani
    • Koji Teramoto
    • Yoshitomo Ozaki
    • Satoru Sawai
    • Noriaki Tezuka
    • Hideaki Ishida
    • Kiichi Kajino
    • Shozo Fujino
    • Akira Yamauchi
    • Osamu Taguchi
    • Reiji Kannagi
    • Hiroyasu Yokomise
    • Kazumasa Ogasawara
  • View Affiliations

  • Published online on: April 1, 2007     https://doi.org/10.3892/or.17.4.895
  • Pages: 895-902
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Abstract

In order to achieve sufficient therapeutic potency, it has been proposed that vaccine therapy with dendritic cells needs to be combined with manipulation of immunological checkpoints, such as inhibition of regulatory T cells and blockade of negative signals, and enhancement of T cell trafficking to tumor sites. In the combinatorial cancer immunotherapy, use of matured/activated dendritic cells (DCs) with more potent antigen presenting capacity seems to be essential for eliciting anti-tumor immune responses. We herein established an ex vivo induction strategy for activated DCs capable of eliciting efficient tumor antigen-specific cytotoxic T lymphocytes (CTLs) from patients with metastatic cancer as well as healthy donors. Immature DCs were matured by 48-h culture in the presence of anti-CD40 antibody and penicillin-killed streptococcus pyogenes (OK432). Supplementation with both anti-CD40 and OK432 resulted in induction of activated DCs with higher surface expression of CD80, CD83, CD86 and major histocompatibility complex class II antigens, compared with other mature DCs that were induced by the combination of anti-CD40 with tumor necrosis factor-α or lipopolysaccharide. In analysis of the produced cytokine profiles, the activated DCs produced the highest T-helper 1-type cytokines for at least 72 h. Furthermore, the activated DCs, pulsed with tumor-associated antigen peptide, elicited in vitro tumor-specific CTLs, but DCs activated with other combinations did not in cancer patients. Therefore, we suggest that the activated DCs studied here might be used as a basic element for the combinatorial cancer immunotherapy.

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April 2007
Volume 17 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kontani K, Teramoto K, Ozaki Y, Sawai S, Tezuka N, Ishida H, Kajino K, Fujino S, Yamauchi A, Taguchi O, Taguchi O, et al: Preparation of fully activated dendritic cells capable of priming tumor-specific cytotoxic T lymphocytes in patients with metastatic cancer using penicillin-killed streptococcus pyogenes (OK432) and anti-CD40 antibody. Oncol Rep 17: 895-902, 2007
APA
Kontani, K., Teramoto, K., Ozaki, Y., Sawai, S., Tezuka, N., Ishida, H. ... Ogasawara, K. (2007). Preparation of fully activated dendritic cells capable of priming tumor-specific cytotoxic T lymphocytes in patients with metastatic cancer using penicillin-killed streptococcus pyogenes (OK432) and anti-CD40 antibody. Oncology Reports, 17, 895-902. https://doi.org/10.3892/or.17.4.895
MLA
Kontani, K., Teramoto, K., Ozaki, Y., Sawai, S., Tezuka, N., Ishida, H., Kajino, K., Fujino, S., Yamauchi, A., Taguchi, O., Kannagi, R., Yokomise, H., Ogasawara, K."Preparation of fully activated dendritic cells capable of priming tumor-specific cytotoxic T lymphocytes in patients with metastatic cancer using penicillin-killed streptococcus pyogenes (OK432) and anti-CD40 antibody". Oncology Reports 17.4 (2007): 895-902.
Chicago
Kontani, K., Teramoto, K., Ozaki, Y., Sawai, S., Tezuka, N., Ishida, H., Kajino, K., Fujino, S., Yamauchi, A., Taguchi, O., Kannagi, R., Yokomise, H., Ogasawara, K."Preparation of fully activated dendritic cells capable of priming tumor-specific cytotoxic T lymphocytes in patients with metastatic cancer using penicillin-killed streptococcus pyogenes (OK432) and anti-CD40 antibody". Oncology Reports 17, no. 4 (2007): 895-902. https://doi.org/10.3892/or.17.4.895